Phospho-ERK1/2 (T202/Y204 / T185/Y187) / Total In-Cell ELISA Kit (Chemiluminescent) [Luminescent]

[CAT#: NPP2011ZP291]

Applications:: ELISA; In-Cell ELISA

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Product Overview

Description

Phospho-ERK1/2 (T202/Y204 + T185/Y187) + Total In-Cell ELISA Kit (Chemiluminescent) provides a simple, efficient, cell-based method for detecting phosphorylated activated protein. This kit is specifically designed to quantify phosphorylated ERK1/2 and/or total ERK1/2. Cells are cultured in 96-well plates and stimulated to induce pathways of interest. After stimulation, cells are quickly fixed to preserve activation-specific protein modifications. Each well is then incubated with a primary antibody that can recognize phosphorylated ERK1/2 or total ERK1/2. Subsequent incubation with hrp-labeled secondary antibody and developing solution provides a chemiluminescence readout that is easy to quantify. Then use the provided crystal violet solution to determine the relative number of cells per well. The 96-well plate format is suitable for high-throughput screening applications.

Species Reactivity

Human

Applications

ELISA; In-Cell ELISA

Pathways

Kinase

Properites

Platform

Microplate

Detection Method

Luminescent

Sample Type

Adherent cells, Suspension cells

Assay Type

Cell-based (quantitative)

Assay Duration

Multiple steps standard assay

Preparation and Storage

Storage

Store at +4°C. Please refer to protocols.

Research Use Only

For research use only

Target Details

Target

ERK2

Official Name

MAPK1

Full Name

mitogen-activated protein kinase 1

Alternative Names

MAPK 2; MAPK 1; MAP kinase 2; ERK-2; p41; ERK; MAP kinase 1; PRKM2; PRKM1; EC 2.7.11.24; MAPK2; p40; Extracellular signal-regulated kinase 2; p38; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 1; ERK2; MAP kinase isoform p42; p42-MAPK; P42MAPK; p41mapk; ERT1