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Creative Biolabs
Product

Human eNOS ELISA Kit [Colorimetric]

[CAT#: NPP2011ZP79]

Applications:
ELISA

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Product Overview

Description

The Human eNOS ELISA Kit is a single wash 90 min sandwich ELISA for the quantitative determination of eNOS protein in cell lysates. It uses our proprietary SimpleStep ELISA® technology. The sensitivity of 77pg/ml of human eNOS was measured.

Species Reactivity

Human

Applications

ELISA

Research Areas

Neurotransmission

Pathways

Nitric Oxide Signaling

Marker

Nitric Oxide
Properites

Sensitivity

77 pg/ml

Platform

Microplate

Detection Method

Colorimetric

Sample Type

Cell Lysate

Assay Type

Sandwich (quantitative)

Assay Time

1h 30m

Assay Duration

One step assay

Linear Range

234.38 pg/ml - 15000 pg/ml

Components

Components 1 x 96 tests
10X Human eNOS Capture Antibody 1 x 600µl
10X Human eNOS Detector Antibody 1 x 600µl
10X Wash Buffer PT 1 x 20ml
50X Cell Extraction Enhancer Solution 1 x 10ml
5X Cell Extraction Buffer PTR 1 x 1ml
Antibody Diluent CPR 1 x 6ml
Human eNOS Lyophilized Recombinant Protein 2 vials
Plate Seals 1 unit
Sample Diluent NS 1 x 12ml
SimpleStep Pre-Coated 96-Well Microplate 1 unit
Stop Solution 1 x 12ml
TMB Development Solution 1 x 12ml
Preparation and Storage

Storage

Store at +4°C. Please refer to protocols.

Research Use Only

For research use only
Target Details

Target

NOS3

Official Name

NOS3

Full Name

nitric oxide synthase 3 (endothelial cell)

Alternative Names

Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 1.14.13.39; ECNOS

Gene ID

4846(Human);18127(Mouse)

Uniprot ID

P29474(Human);P70313(Mouse)
Publications

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For Research Use Only. Not For Clinical Use.
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