Creative Biolabs

Human iPSC-Derived Neural Stem Cells (Female), Source Cord Blood CD34+ Cells

[CAT#: NCL-2101-ZP03]

Please be aware that the correct volume of Neural Plating Medium (Cat. NCC-2101-ZP07) (not Xeno-Free) needed for your specific order volume of cells will be supplied FREE OF CHARGE.

Species:
Human
Applications:
Cell Assay
Cell Types:
Neural Stem Cell

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Description
Creative Biolabs human iPSC-derived neural stem cells (NSC) are derived from integration-free, induced pluripotent stem cells (iPSC) under fully defined neural induction conditions. These NSCs express typical markers of cerebral cortex neural stem and progenitor cells, such as PAX6 , FOXG1 and Nestin, and when they are laid in a single layer, they will spontaneously form a polarized neural tube rosette structure. In addition, Creative Biolabs NSC can produce a series of cerebral cortex excitatory and inhibitory neurons, these neurons have electrical activity And has the ability to form functional synapses and circuits in vitro. After thawing and plating, neural stem cells finally differentiate to obtain mature electrophysiological properties, and form a functional synaptic network within 40 to 50 days.

Following our protocols and using our tailor-made media and reagent packs, Creative Biolabs NSC can be easily distinguished into neurons or mixed neuronal cell types. After synchronous differentiation, highly purified neurons can be produced from Creative Biolabs NSC. Using our special coating reagent, it is possible to culture neurons from Creative Biolabs NSC for a long period of time (> 1 year). NSC can be obtained from multiple donors to meet your research needs and has a wide range of characteristics.
Cell Types
Neural Stem Cell
Applications
Cell Assay
Species
Human
Size
1.5 million cells & Neural Plating-XF Medium
Growth Pattern
Adherent
Tissue Source
Cord Blood CD34+ Cells
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
vapour phase nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.
For Research Use Only. Not For Clinical Use.
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