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Creative Biolabs
Product

Human Neuron specific Enolase ELISA Kit [Colorimetric]

[CAT#: NPP2011ZP18]

Applications:
ELISA

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Product Overview

Description

Human Neuron Specific Enolase ELISA Kit is a single wash 90 min sandwich ELISA for cell culture extracts, cell culture supernatants, cit plasma, edta plasma, hep plasma, serum and tissue extracts. Quantitative determination of meta-specific enolase protein. It uses our proprietary SimpleStep ELISA® technology. The sensitivity of human neuron-specific enolase at 23 pg/ml was measured.

Species Reactivity

Human

Applications

ELISA

Research Areas

Neural Cell

Marker

Soma
Properites

Sensitivity

23 pg/ml

Platform

Microplate

Detection Method

Colorimetric

Sample Type

Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma

Assay Type

Sandwich (quantitative)

Assay Time

1h 30m

Assay Duration

One step assay

Linear Range

312.5 pg/ml - 20000 pg/ml

Components

Components 1 x 96 tests
10X Human Neuron-specific Enolase Capture Antibody 1 x 600µl
10X Human Neuron-specific Enolase Detector Antibody 1 x 600µl
10X Wash Buffer PT 1 x 20ml
50X Cell Extraction Enhancer Solution 1 x 1ml
5X Cell Extraction Buffer PTR 1 x 10ml
Antibody Diluent CPI - HAMA Blocker 1 x 6ml
Human Neuron-specific Enolase Lyophilized Recombinant Protein 2 vials
Plate Seals 1 unit
Sample Diluent NS 1 x 50ml
SimpleStep Pre-Coated 96-Well Microplate 1 unit
Stop Solution 1 x 12ml
TMB Development Solution 1 x 12ml
Preparation and Storage

Storage

Store at +4°C. Please refer to protocols.

Research Use Only

For research use only
Target Details

Target

NSE

Official Name

ENO2

Full Name

enolase 2 (gamma, neuronal)

Alternative Names

Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC 4.2.1.11

Gene ID

2026(Human);13807(Mouse)

Uniprot ID

P09104(Human);P17183(Mouse)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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