Tel:
Fax:
Email:
Creative Biolabs
Product

Human Syndecan-2(SDC2) ELISA kit

[CAT#: NRZP-0722-ZP228]

For the quantitative determination of human syndecan-2 (SDC2) concentrations in serum, plasma, tissue homogenates.

Datasheet MSDS Request COA

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry
Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human SDC2.

Species Reactivity

Human

Marker

Syndecan 2

Principle

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SDC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDC2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Properites

Sensitivity

23.43 pg/ml

Detection Method

Sandwich

Sample Type

serum, plasma, tissue homogenates

Assay Type

quantitative

Assay Time

3-5 working days

Assay Duration

1-5h

Precision

Intra-assay Precision (Precision within an assay): CV%<8%.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.

Number Of Samples

50-100ul

Detection Range

93.75 pg/ml-6000 pg/ml

Max Emission

450 nm
Target Details

Target

SDC2

Official Name

SDC2

Full Name

syndecan 2

Alternative Names

HSPG, HSPG1, SYND2, cell surface-associated heparan sulfate proteoglycan 1, fibroglycan, heparan sulfate proteoglycan 1, cell surface-associated, heparan sulfate proteoglycan core protein, syndecan prot

Gene ID

6383(Human)

Uniprot ID

P34741(Human)
Protocols

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
Publications

Publications (0)

gift-card

Related Products
For Research Use Only. Not For Clinical Use.
Fill out this form for a quote Inquiry Form Send Inquiry
Inquiry Basket
compare

Send inquiry