Human Vitamin D Receptor, VD R ELISA Kit

[CAT#: NRZP-0722-ZP265]

For the quantitative determination of human vitamin D receptor (VD R) concentrations in serum, plasma, tissue homogenates, cell lysates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human VD R.

Species Reactivity

Human

Marker

Vitamin D (1,25- dihydroxyvitamin D3) receptor

Properites

Sensitivity

In most cases, the sensitivity is below 1.56 pg/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, tissue homogenates, cell lysates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

6.25 pg/ml-400 pg/ml

Max Emission

450 nm

Target Details

Target

VDR

Official Name

VDR

Full Name

vitamin D receptor

Alternative Names

NR1I1, vitamin D (1,25-dihydroxyvitamin D3) receptor; PPP1R163

Gene ID

7421(Human)

Uniprot ID

P11473(Human)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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