iNeu™Cal™ Calcium Biosensor Assay using Human Neural Progenitor Cells

[CAT#: NCL-2103-P61]

Species:: Human

Applications:: Calcium Assay

Cell Types:: Neural Progenitor Cells

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Product Overview

Description

iNeuCal™ calcium biosensor has been integrated into human neural progenitor cells. They respond to changes in intracellular calcium in the 605nm-625nm fluorescence range (excitation/emission = 610nm / 625nm) to measure the increase or decrease in intracellular calcium concentration. These calcium biosensors have an excellent signal-to-noise ratio and can operate in the range of seconds to minutes. They can be used in single cell applications as well as medium to high throughput screening platforms.

Cell Types

Neural Progenitor Cells

Applications

Calcium Assay

Application Notes

Applications:

Neurotoxicity testing for reporting cellular calcium fluctuations
Chemical compound or small molecules testing or screening
Research and Development on animal models
Studying neurogenesis, neuronal differentiation and function, and calcium-mediated signal transduction pathways.

Species

Human

Properties

Size

~0.6x10^6 cells per 1ml of freezing medium (vial)

Growth Pattern

adherent

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.