Product

Lenti of Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein (NM_026880) ORF clone, Myc-DDK Tagged

[CAT#: NEP-0621-R0148]

; The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.;

Target:: PINK1

Gene/Insert name:: PINK1

Type:: Mouse Tagged ORF Clone

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Product Overview

Description

This is a mouse PINK1 Myc-DDK tagged ORF clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

PINK1

Species

Mouse

Insert Size (bp)

1740 bp

Tag

Myc-DDK

Type

Mouse Tagged ORF Clone

Vector

pLenti-C-Myc-DDK

Cell Selection

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGGCGGTGCGACAGGCACTGGGCCGAGGCCTGCAGCTGGGTCGGGCGCTGCTGCTGCGCTTCGCGCCCAAGCCCGGCCCGCTGTTCGGCTGGGGGAAGCCCGGCCCCGCGGCGGCCTGGGGCCGCGGAGAGCGCCCAGGCCAGGTCGTAAGCCCCGGAGCACAGCCTCGGCCGGTCGGGCTCCCCCTCCCGGACCGCTACCGCTTCTTCCGCCAGTCGGTAGCTGGGCTGGCGGCGCGGATCCAGAGGCAGTTCATGGTGCGGGCCCGGGGCGGCGCAGGCCCTTGCGGCCGAGCGGTCTTCCTGGCCTTCGGGCTGGGGCTGGGGCTGATCGAGGAGAAGCAGGCGGAAGGCCGGAGGGCCGCCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACACAGAAAACCAAGCGCGTGTCTGACCCACTGGACACTCGATGCTGGCAGGGCTTCCGTCTGGAGGATTATCTGATAGGGCAAGCCATTGGCAAGGGTTGCAATGCCGCTGTGTATGAAGCCACCATGCCCACGCTGCCCCAGCACCTGGAAAAGGCCAAACACCTTGGCCTTATAGGAAAGGGCCCGGATGTCGTCCTGAAGGGAGCAGACGGGGAGCAGGCTCCAGGGACTCCCACCTTTCCCTTTGCCATCAAGATGATGTGGAATATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTAAGCAAAATGAGCCAGGAGCTGGTCCCGGCAAGCCGCGTGGCTTTGGCTGGAGAGTATGGAGCAGTTACTTACAGAAGATCCAGAGATGGTCCCAAGCAGCTTGCCCCACACCCTAACATCATCCGGGTTTTCCGCGCCTTCACCTCATCTGTGCCCCTCCTGCCGGGGGCCCTGGCTGACTATCCTGATATGCTGCCCCCACACTACTACCCAGAAGGCCTGGGCCACGGTCGCACACTGTTCCTCGTTATGAAGAACTACCCCTGTACCCTGCGCCAGTACCTTGAGGAGCAGACTCCCAGTTCTCGCCTGGCTACCATGATGACCTTGCAGTTGCTGGAGGGCGTGGACCATCTGGTTCAGCAGGGCATTGCCCATCGGGATCTCAAGTCCGACAACATCCTTGTGGAGTGGGACTCAGATGGCTGTCCCTGGCTAGTGATCTCAGACTTTGGCTGCTGCCTGGCTGACCAGCATGTTGGCCTGCGGCTGCCTTTCAACAGCTCCAGTGTAGAGCGTGGTGGCAATGGCTCCCTGATGGCCCCTGAGGTGTCCACAGCCCATTCTGGCCCCAGTGCGGTAATTGACTACAGCAAAGCCGATACCTGGGCTGTGGGGGCCATCGCCTATGAAATCTTTGGGCTTGCCAATCCCTTCTATGGCCAAGGCAGTGCCCACCTCGAGAGCCGCAGCTACCAGGAAGCTCAGCTGCCTGAGATGCCTGAGTCGGTGCCTCCAGAGGCAAGACGGCTGGTGAGGTCACTGCTCCAGCGAGAGGCCAGCAAGAGACCGTCTGCACGCTTAGCTGCAAATGTGCTGCACTTAAGCCTCTGGGGCGAGCATCTTCTAGCCCTGAAGAACCTGAAATTGGACAAGATGATCGCCTGGCTCTTGCAGCAGTCAGCAGCCACTCTGCTGGCTGACAGGCTGAGAGAGAAGAGCTGCGTGGAGACAAAGCTGCAGATGCTGTTTCTGGCTAACCTGGAGTGTGAGGCTCTCTGCCAGGCAGCCCTCCTCCTCTCTTCCTGGAGGGCAGCCCCAACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA

ORF Nucleotide Sequence

MAVRQALGRGLQLGRALLLRFAPKPGPLFGWGKPGPAAAWGRGERPGQVVSPGAQPRPVGLPLPDRYRFFRQSVAGLAARIQRQFMVRARGGAGPCGRAVFLAFGLGLGLIEEKQAEGRRAASACQEIQAIFTQKTKRVSDPLDTRCWQGFRLEDYLIGQAIGKGCNAAVYEATMPTLPQHLEKAKHLGLIGKGPDVVLKGADGEQAPGTPTFPFAIKMMWNISAGSSSEAILSKMSQELVPASRVALAGEYGAVTYRRSRDGPKQLAPHPNIIRVFRAFTSSVPLLPGALADYPDMLPPHYYPEGLGHGRTLFLVMKNYPCTLRQYLEEQTPSSRLATMMTLQLLEGVDHLVQQGIAHRDLKSDNILVEWDSDGCPWLVISDFGCCLADQHVGLRLPFNSSSVERGGNGSLMAPEVSTAHSGPSAVIDYSKADTWAVGAIAYEIFGLANPFYGQGSAHLESRSYQEAQLPEMPESVPPEARRLVRSLLQREASKRPSARLAANVLHLSLWGEHLLALKNLKLDKMIAWLLQQSAATLLADRLREKSCVETKLQMLFLANLECEALCQAALLLSSWRAAPTRTRPLE - GFP Tag - V

Protein Sequence

SgfI-MluI

Application Notes

Ion-exchange column purified

Purification

Neural Diseases

Research Areas

Metabolism

Properties

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at-20°C. The DNA is stable for at least one year from date of shipping when stored at-20°C

Research Use Only

For research use only

Target Details

Target

PINK1

Official Name

PINK1

Full Name

PTEN induced putative kinase 1

Alternative Names

BRPK;
FLJ27236;
mitochondrial;
PARK 6;
PARK6;
Phosphatase and Tensin Homolog;
PINK 1;
PINK1;

Gene ID

65018(Human)

Uniprot ID

Q9BXM7(Human)

References

1. Yin, F., Yan, J., Zhao, Y., Guo, K. J., Zhang, Z. L., Li, A. P., ... & Guo, L. (2019). Bone marrow mesenchymal stem cells repair Cr (VI)-injured kidney by regulating mitochondria-mediated apoptosis and mitophagy mediated via the MAPK signaling pathway. Ecotoxicology and environmental safety, 176, 234-241. 2. Espinoza-Derout, J., Shao, X. M., Bankole, E., Hasan, K. M., Mtume, N., Liu, Y., ... & Friedman, T. C. (2019). Hepatic DNA damage induced by electronic cigarette exposure Is associated with the modulation of NAD+/PARP1/SIRT1 axis. Frontiers in Endocrinology, 10, 320. 3. Zhang, Z., Chen, Z., Liu, R., Liang, Q., Peng, Z., Yin, S., ... & Liu, Y. (2020). Bcl-2 Proteins Regulate Mitophagy in Lipopolysaccharide-Induced Acute Lung Injury via PINK1/Parkin Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2020.

Publications