Lenti of Rat neurofilament, medium polypeptide (Nefm) (NM_017029) ORF clone, mGFP Tagged

Description

This is a rat NEFM mGFP tagged ORF clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

NEFM

Species

Rat

Insert Size (bp)

2535 bp

Tag

mGFP

Type

Expression Vector/ Viral Particle

Vector

pLenti

Vector Type

CMV

Virus Type

CMV

E. coli Selection

Chloramphenicol (34 ug/mL)

Cell Selection

Puromycin

ORF Nucleotide Sequence

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGAGCTACACGCTGGACTCGCTGGGCAACCCGTCCGCCTACCGGCGCGTCACCGAGACCCCGTCCAGCTTCAGTCGTGTGAGCGGTTCCCCGTCCAGCGGCTTCCGCTCGCAGTCCTGGTCCCGCGGCTCGCCCAGCACCGTGTCCTCCTCCTACAAGCGCAGCGCGCTCGCCCCGCGCCTCGCCTACAGCTCGGCTATGCTCAGCTCGGCCGAGAGCAGCCTCGACTTCAGCCAGTCCTCTTCGCTGCTTAACGGCGGCTCCGGCGGCGACTACAAGCTGTCCCGCTCAAACGAGAAAGAGCAGCTGCAGGGGCTGAACGACCGTTTCGCCGGCTACATCGAGAAAGTGCACTACTTGGAACAACAGAACAAGGAGATCGAGGCAGAGATCCACGCGCTGCGGCAGAAGCAGGCCTCGCACGCCCAGCTGGGTGACGCTTACGACCAGGAGATCCGAGAGCTGCGCGCCACCCTGGAGATGGTGAATCACGAGAAGGCTCAAGTGCAGCTGGACTCTGATCACTTGGAGGAAGACATCCACCGGCTCAAGGAGCGCTTCGAGGAGGAGGCGCGGCTGCGGGACGACACCGAGGCTGCCATCCGGGCGCTGCGCAAAGACATAGAGGAGTCGTCGATGGTTAAGGTGGAGCTGGACAAGAAGGTGCAGTCGCTGCAGGATGAGGTGGCCTTCCTGCGGAGCAATCACGAAGAGGAGGTGGCCGACCTGCTGGCCCAGATCCAGGCGTCGCACATCACCGTAGAGCGCAAAGACTACCTGAAGACAGACATCTCCACGGCGCTGAAAGAGATCCGCTCCCAGCTCGAGTGTCACTCCGACCAGAACATGCACCAGGCCGAAGAGTGGTTCAAATGCCGCTACGCCAAGCTCACCGAGGCGGCCGAGCAGAACAAGGAGGCCATCCGCTCCGCTAAAGAAGAGATCGCCGAGTACCGGCGCCAGCTGCAGTCCAAGAGCATTGAGCTCGAGTCGGTGCGAGGCACTAAGGAGTCCCTGGAACGGCAGCTCAGCGACATCGAGGAGCGCCACAACCACGACCTCAGCAGCTACCAGGACACCATCCAGCAGCTGGAAAATGAGCTTCGGGGAACAAAGTGGGAAATGGCTCGTCATTTGCGAGAATACCAGGATCTCCTTAACGTCAAGATGGCTCTGGACATCGAGATCGCCGCATATAGGAAACTACTGGAGGGTGAAGAGACCAGATTTAGCACATTTTCAGGAAGCATCACTGGGCCTCTGTACACACACCGACAGCCCTCAGTCACAATATCCAGTAAGATTCAGAAGACCAAAGTCGAGGCCCCCAAGCTCAAGGTCCAACACAAATTTGTGGAGGAGATCATTGAGGAGACTAAAGTGGAAGATGAGAAGTCAGAAATGGAAGACGCCCTCACAGTCATTGCAGAGGAATTGGCAGCCTCTGCCAAAGAGGAGAAAGAAGAGGCAGAAGAAAAGGAAGAGGAACCGGAAGTTGAAAAGTCTCCCGTGAAGTCTCCTGAGGCTAAGGAAGAGGAGGAAGGGGAAAAGGAGGAAGAAGAGGAAGGCCAAGAGGAAGAAGAGGAGGAAGATGAAGGTGTCAAGTCAGACCAGGCAGAAGAGGGAGGATCTGAGAAGGAAGGCTCGAGTGAAAAGGATGAAGGTGAGCAAGAAGAAGAAGGGGAAACTGAGGCAGAAGGTGAAGGAGAGGAAGCAGAAGCTAAGGAGGAAAAGAAAACAGAGGGAAAGGTCGAGGAAATGGCTATCAAGGAGGAAATCAAGGTCGAGAAGCCCGAGAAAGCCAAGTCCCCTGTGCCAAAATCACCCGTGGAAGAAGTAAAGCCAAAACCAGAAGCCAAAGCCGGAAAGGATGAGCAGAAGGAGGAAGAGAAAGTTGAGGAGAAGAAGGAGGTAGCCAAGGAATCACCCAAGGAAGAGAAGGTGGAGAAAAAGGAGGAGAAGCCAAAAGATGTCCCAGATAAAAAGAAGGCTGAGTCCCCAGTGAAAGAAAAGGCCGTAGAGGAAATGATCACCATTACTAAGTCGGTAAAGGTGAGCCTGGAGAAAGACACCAAAGAGGAGAAGCCTCAGCAGCAGGAGAAGGTGAAGGAGAAGGCAGAGGAGGAGGGGGGTAGTGAGGAGGAAGTGGGTGACAAAAGCCCGCAAGAATCCAAGAAGGAAGACATAGCTATCAATGGGGAGGTGGAAGGAAAAGAGGAGGAGGAGCAGGAAACTCAGGAGAAGGGCAGTGGGCAAGAGGAGGAGAAAGGGGTGGTCACTAATGGCTTAGATGTGAGCCCTGCGGAGGAAAAGAAAGGGGAGGATAGAAGTGATGACAAAGTGGTGGTGACCAAGAAGGTAGAAAAAATCACCAGCGAGGGAGGCGATGGTGCTACCAAATACATCACCAAATCTGTTACTGTCACTCAAAAGGTTGAAGAGCATGAGGAGACCTTTGAGGAGAAGCTGGTGTCAACTAAAAAGGTAGAAAAGGTCACTTCACATGCCATAGTCAAGGAAGTCACCCAGGGTGACACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA

Restriction Sites

SgfI-MluI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neuron; Neurotransmitter

Relevant Diseases

Alzheimer's Disease; Neurological Disorders

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at-20°C. The DNA is stable for at least one year from date of shipping when stored at-20°C

Research Use Only

For research use only

Target

NEFM

Official Name

NEFM

Full Name

Neurofilament medium polypeptide

Alternative Names

NEFM; NEF3; NF-M; NFM; neurofilament; medium polypeptide; neurofilament medium

Gene ID

4747(Human)

Uniprot ID

P07196(Human)