Mouse cyclin-dependent kinase 5, regulatory subunit (p35) 1 (cDNA clone MGC:65701 IMAGE:6825809) (BC058697) ORF clone, Untagged

Description

This is a mouse CDK5R1 untagged clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

CDK5R1

Species

Mouse

Insert Size (bp)

924 bp

Tag

Tag Free

Type

Expression Vector/ Viral Particle

Vector

pCMV6

Vector Type

CMV

Virus Type

CMV

E. coli Selection

Kanamycin (25 ug/mL)

Cell Selection

Neomycin

ORF Nucleotide Sequence

GGAGCCGCCGCCGCGCCGAGTCGAGCACCCGGGCCGCGGCGCGCATTGCGGAGGGCCTGGGACGCAGCGG CTGCGCCTGCCGCGGGAGCCTGCCTGACTGCGGGTAAGCCTGCGCGGACCGGGGCCGTGTGCGCGGCCTG GTTGGATTCCTGCTGCGGAGGCCACTGCGGCTCTTTGACCGGTGCCGCAGTGGAAGCACAGGCTGGGGCT CGACGGGGCCGCAGCCCGGGGGTTCGGGCCTCGGAAGTGCGAGCGGAGGGGCGCGGCGTGGAGCCCCGCC CGGGGCTGAGAACCATCTTGTTTTCCACGCAGATCCGCAGGACTAAACGCGTCGTGTCCCGGGAGCGCCT CCTTTCTCTCTGCGGCCGCTACAGGCTCGGTAAGCCTTTACCCCCCTCCAGCCGGCAGGCTGGGCGCGCG GGGGTGCCGGCCCCTGCCGGGCGCGCAGTGGCACCATGGGCACGGTGCTGTCCCTATCCCCCAGCTATCG GAAGGCCACACTGTTTGAGGATGGCGCGGCCACCGTGGGCCACTACACGGCCGTGCAGAACAGCAAGAAC GCCAAGGACAAGAACCTGAAGCGGCACTCCATCATCTCGGTGCTGCCTTGGAAGAGGATCGTGGCGGTGT CAGCGAAGAAGAAGAACTCCAAGAAGGCGCAGCCCAACAGCAGCTACCAGAGCAACATCGCGCATCTCAA CAATGAGAACCTGAAGAAGTCGCTGTCCTGTGCCAACCTGTCCACATTCGCCCAGCCCCCGCCGGCTCAG CCGCCCGCACCCCCAGCCAGCCAGCTTTCTGGCTCCCAGACTGGGGTCTCCTCTTCTGTCAAGAAGGCCC CGCACCCTGCCATCACCTCTGCAGGGACACCCAAACGGGTCATCGTCCAGGCGTCCACTAGTGAGCTGCT GCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCTACCGCCTGAAGCACTTGTCCCCAACGGACCCCGTG CTCTGGCTGCGCAGCGTGGACCGCTCCCTGCTTCTGCAGGGCTGGCAGGACCAGGGTTTCATCACACCGG CCAATGTGGTCTTCCTCTACATGCTCTGCAGGGATGTCATCTCCTCTGAGGTGGGCTCCGACCATGAGCT CCAGGCTGTCCTGCTGACCTGTCTGTACCTCTCCTATTCCTACATGGGCAATGAGATCTCCTACCCGCTC AAGCCCTTCCTGGTAGAGAGCTGTAAGGAAGCCTTTTGGGACCGTTGCCTCTCAGTTATCAACCTCATGA GCTCCAAGATGCTGCAGATCAATGCCGACCCACACTATTTCACACAAGTGTTCTCTGACTTGAAGAATGA GAGCGGTCAGGAGGACAAGAAGCGACTCCTCCTGGGGCTGGATCGGTGAGCCCCTGTAGCCGGTATCCTG GCTCAAGGATTCCACTTGTTTTTAAATTTTATTACTAAAAGAATCAAGTTTTGTGTACAGTATGTGTCTA GCAGAGCCACCAAGGGCCTCTTCCCACGTTCTTTCCTTGGGGTTTTTTTTTAGCCCTTTCAAGAACTCTG GGGACTTTCGAACTGATGAACCTTGCGCGAACCCAAAAGATGCATTCAGAGCCACTTCGGCTGCTGACCA CTCACTTTCCGGGACTCAAGGGACTGACTTGCCCATGTCACCTGTACCCACGATGGATCCAGGGTAGATG AGGCTTCCCACTGTCCCATGATCGGAGCTGGGGCCCATGGCTGGTGGCCCTGGAGGAATCAGCCCTTTTC TGGGTATTGTTTCTCTTGAAGGCTCCTGGAGCTGGCTGTCCTGACTCAGGATCCAGGAGGTTGTGAGTGA GGCAAGATCTGCACAGCCCTGGGGCATCAGTGCAGGAGCCTGCAGAGACTGCTGATGACTGTTGACCATG CACGGCCCTGGTTTGCTGCGTGCTTTTTTAGGGAGAAGGGCTTTCCTTTCCTGGCAGGACAGAGCTCCCC TCCCATCCTTTTGTCTCTGCTCCCACGTTAGTGCTGGTTAATGAGGTGGTTTGCTCATTAGATTCCTCAC CCGTTTTGGGTCCCAGCTAAAGGATGAGGTGAAGCTAGGGGCAGCTCCTTCCCTGAGCGAGCTTTTCACA GTTTGTACCCTGTGTACAAAGCCAGAACAGCGCCGGGACCCACTAACAACATTGCCTTCTGGGCGGGTGG GTGTCTATGCCTGAGATCAGGTTGGTGCCCACTTGCATGCTAAGGCCTCTCGCTGGTGCCCGTGATCTTT GTTCTGTATCGTTGGGGGGAGGTGAGATCAGTCAATAGGGGTGTTGCTGTTTCCCATCCCTGCCCCCCCC CCAATTGTTAATTTTAAGCTGTTTCCAGGCAGCTCCGTCTCTTCCTGGAGAGGCTTTGCTCTGTGCAACC TACTGAGACAGATGCATTTGGGGACAATTACACGATGGAAGTGTGACCCCTGATCAGTTTGGAAGCTTGG TGACTGTGCAGCTGAGCACTTCCAGCTGCAGGGTGTGGCCGCCCACCTATCCCTGTCAGCTGCCTAACAC ACTCTGAACTTTAAGGGTTTTTCTTTCTAGTTTTCCCAGACTGGTGGCTTGGGGTTTGGAAGTGGTCCAA GAGAAGGGAGCTAGTCTTCTTGCATGGCCCTCACTCCAGCTTTGGGGAACAAAGAGCAATGTGAAGATAC TGCGGAGGGTTTCCTCTTCCTCTCCTGCTGCCCACCCCCGGGGGGGGGTGTGGGGGGCTGGGGCTGGGGC TGGGGGCTACAGTGAGGAAGGCGGAGGTCATCTTGCATAGGATTAGGTTCTAGGTGACTGCCAACTTCTT GCACAAGCACTACTGAAAAATTGCATATAAAGATAAAGCTGTGAAATTGAGGTCTCAATGGAAGAGGCCC GGAGCAGAGCCTTTCAGCCGACGACGTACTCACAGAGGTGGGGAAGCGGACCTGACAGACTAGAGAGCCT TTCCCCGGCCCCTCATCCGGGTTCCTAGAGAGCCGATCCTGGGCCCTCAGATGGTCTGACACGGAGCTGG AAAGCCCTGCAAGGCTTGCTGAGTGCAGGCCTCGCTGCAGCCCATCCTCACATCCTGCTGCCACGGTGAC CGCGCCTGGAGCCCCATGGGGCAGTAGGGAGAAGACCTTCAGGTGTCCAGTCTAAGCAGGAGCGGGCTCA TCCTCATAGCCTGCCAGCCGCTGTCGCTGGTCCACTGAGCTGCGGTAACAGGATTCCAGCTTTGCTGACT GTTTCCTTCTCTTTGTACTTAATTTTTAAAAAACAAAACAAAACATTTTTTTTGAGAAGGTGCATATTTC AGAAGTGGTCGGCAACTGAGGAAAGAGCTTCCTTGGTTCCATTGTGAAGCCCGTGTCCTCTCTGCTTGCG CTGGCTCTCAGTACTGTGTAAAGGAACGACCGCTAGACTTAAGTGTTCGAGCAAAGAACCTATCTGACAT GCTGCTATGAAGACACTTTTAGAACACACACACAAGATAGAAAAGAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Restriction Sites

AscI-NotI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neural Cell; Neuron

Relevant Diseases

Neurodegenerative Disease; Alzheimer's Disease

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C

Research Use Only

For research use only

Target

p35

Official Name

CDK5R1

Full Name

Cyclin-dependent kinase 5 activator 1

Alternative Names

CDK5R1; CDK5P35; CDK5R; NCK5A; p23; p25; p35; p35nck5a; cyclin-dependent kinase 5; regulatory subunit 1 (p35); cyclin dependent kinase 5 regulatory subunit 1

Gene ID

8851(Human); 12569(Mouse); 116671(Rat)

Uniprot ID

Q15078(Human); P61809(Mouse); P61810(Rat)