Homo sapiens, clone IMAGE:4939043 (BC035448) ORF clone, Untagged

Description

This is a human CDK5R1 untagged clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

CDK5R1

Species

Human

Insert Size (bp)

3800 bp

Tag

Tag Free

Type

Expression Vector/ Viral Particle

Vector

pCMV6

Vector Type

CMV

Virus Type

CMV

E. coli Selection

Ampicillin (100 ug/mL)

ORF Nucleotide Sequence

GCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCTGCAGGGCTGGCAGGACCAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGCTCTGCAGGGATGTTATCTCCTCCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCTACTCCTACATGGGCAACGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGTTGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACACTACTTCACACAGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTAGGCCTGGATCGGTGAGCACTGTAGCCTGCGTCATGGCTCAAGGATTCAATGCATTTTTAAGAATTTATTATTAAATCAGTTTTGTGTACAGTATGTGTCTAGCAAAGCCACCAAGGGCCTCACCTTTCCCACAGTCTCTCCCTGGGGTTTTTTTCATCCCTGCCAAGAACTCTGGGCACTTTTGAACTCACGAGCCTTGCGCAAAACCCAGAAGATGTATTCAGAGCCACCCAGGCCACTGACCTCCCACTTTGGGGAACTCAAAGGACTGACCTGCCCCTGCCGCCTGTGCCCTTGCTGGGTCCAGGGTAGGCAAGGCTGCCGGCTGCACCCTGTATGGAGCTAGAGAGGGGCCTCTGGCTGCCTGGCCCAGGGAGGAATTGGGGTTTCTGGTTGGAGGCCCTTTTCTGGCTCCTGTGTGGAGTTATTCACTCTCCCAGAGGCTCCTGGAGCCAGCCACCCTAACTGAGCTGCCAGTGGGGTCGTGAGGCAAGATCCCCGCCACCCGGGGACATCTTCAATCTAGGCGAGGCGAAGCTGAGCGGGTCTAGTGGAAAGATTGTGTCTGGTCGTTTGACCACACACCGCCCTGATTTGCTGTTTTCTTTTTTTAGGGAGAAGGGCTTTTCTTTAGTGGAGAAATGGAACTCGCCCCCCTACCCCCTTGTCTGCTGCTCCCAGCCACGTTGGTGGTATTGGCCGATGAGCTGGTTTGACTCATTAATTTCTCTCAATTTGGGTCCCAGCTAAAGAGGTGGGGTGAAGCTGGGGACAGCTTTCCTGGGTGAGTTTTTCTTTTGAATAATGTAGTGCAGTCACCCTGTGGCAAATGCCAGGACAGCTGCAGGTGCCCATTAGCAACGCCTGCCTTCTGGGCAAGGTGAGGGATGCCATACCTTGAGACCAAGCCGGTGCCCACCTAAGGGCTAAGGCCTCTTGCTGGTGCACATGACATTTGTCCTGCAGAGCTGGGGGGAAGGCGATGTGGTGACCCCACCCCCACCCGTTAATTTAAAGCTGTTTCTAAACAGTTGAGTTTCTTCTAAAGAGGAAGCCTTGCCCAGCAAGGACCAGTGAGACAGCCGGATCTTGGAGACAATTACAAGACGGGGAGTGAGACTCCTGATTGCTCTGGAAGCCTGCTGATTTCTACAACTGATCATTTGCAGCTGCTGGTTTTGGTTTCCACCTTACCCTACTGGCTGTAAAAACACAAATGTGTACTTTATTGATTTTCTTTCTAATTCTCCCGCATTGGTGGCTTGGGACTTGGGAGAGGGAGCAAGCCTTCCTCCATGGCCCATCACTCGGCTGTGGAGAACAAAGACCAATGTGAAGACACTACAGAGGATTCTGTCTTCCAGGCCCAGTCCACTGGGGAGTGCTGGAATGGGGACCTGGGGTGGGGAGGCAGAGGGTCACTTTACATAGGATTAAGTTCGAGGTGGCTACCGATTTCAGCACATGCACTACTGAAATTTACACAAAAAGAAAGCTGTGAAATTGAAGTCCCAATTTAAGAGTCCTGAGGCAGAACCTGGTGGCTGGAGGCATTCCCAGAGGTGGGGAAGAGAGCCTGCCCGGCCGGAGAACATCTGCCTTGCTGCACCTGAGGCCCAGCAGAGCCGTTCCTGGGACTGTCAGATAATCGGTGCAGCGGTGGAAGGAGCCTGCGGCTGCTGGCACAGACTTCACACAGCACCTCCTCTCTGCTGGGTTTCCACACAGCCTGTCTTCAGATCCTGCTGCCGCGTGCGACCAGAGGTGGGAGGCCCCTGGTGGCATGGAAGAGGGAGGGTCAGTGCCAAGTCTCAGGAGGAGGGCGCATGTGTGTATCACCCTCAGCTGGCGGAACCTGGCTGCGAACTGTGCAGTTACGTTGCATCCACAGGATTCCAGTTGCGTGTCTGTTTCCTTCTCTTTCTCCGTATTTATTTTTTTATTCTTCGGAGGAGGTGGACATTTCGGAAGTGGTGGGGACTAAGGGAAGAACTCTCTAGTTCCCTCAGTGTGAAGCCTGTCGTGTTCTCTCCCCTTGCACTGGTCATCAGTATTGTGTAAAGGAACAACTGATATACTTGAGTGTGCAAGCAAAGAACCCATTTGCCATGCTGCTATGAAGACTACTTTTAGATCAACAATAAAAAAAAACCTACAAAAAAACCTTTATTCTTTAATTGTTGCTTTTACGGTGATATTGTGCATGCAAACCAGGAGCATTTTGTGTCTTAAGAAAAATAATCTTAGAACAGATGGCTGTGAAAATTACACCCATGCACAGAACAAGCCACAGGAATAATAGTTCAGGATTTGGTTTTTCTCTTTTTCTTGTAAACCTGGAGGGTTGATATATTCTTTCCATGCAGTTATTAGAACTTAGTTTTGTTCCAACAGTTAAACTTGCAATGAAAAGAAAATGTGCCATTTTTTTCACTCAGAATTATTCATAGCTGTATATTTGAAACTGCTAATTACACACGTGTGATGTATGTTGGTTTTTTAGTGCAATTTCTTCTGTAGCTATTCTTTGACCAAACTGTGGGTATTGTTAATATTAATTTATATTTGTCTCATTTTGTATGTATGTGTAGTGTGTTTGTGAGTATGTGTGGTTTATAATCTGACAAAGTCATGAAGCTCAGTTTGGCTGTAATTTAATTCCCCTTCCCTTATTTTTATTTATTTTTGTACTGTGCTGATTCAATAAAATGCACTGACCATCCATAAAAAAAAAAAAAAA

Restriction Sites

NotI-NotI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neural Cell; Neuron

Relevant Diseases

Neurodegenerative Disease; Alzheimer's Disease

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C

Research Use Only

For research use only

Target

p35

Official Name

CDK5R1

Full Name

Cyclin-dependent kinase 5 activator 1

Alternative Names

CDK5R1; CDK5P35; CDK5R; NCK5A; p23; p25; p35; p35nck5a; cyclin-dependent kinase 5; regulatory subunit 1 (p35); cyclin dependent kinase 5 regulatory subunit 1

Gene ID

8851(Human); 12569(Mouse); 116671(Rat)

Uniprot ID

Q15078(Human); P61809(Mouse); P61810(Rat)