Mouse Anti-Neurofilament Heavy Polypeptide Monoclonal Antibody (CBP2419)

Description

Neurofilaments (NF) are ~10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and regulate axon diameter. Neurofilaments belong to the same protein family as the intermediate filaments of other tissues such as keratins, which make the filaments expressed in epithelia. The family of proteins that includes the intermediate filaments is divided into 5 major classes, the keratins forming the classes I and II. Class III contains the proteins vimentin, desmin, peripherin and glial fibrillary acidic protein (GFAP). The major neurofilament subunits occupy the class IV family of intermediate filaments, along with two other filament proteins of neurons, alpha-internexin and nestin. The class IV intermediate filament genes share two unique introns not found in other intermediate filament gene sequences. Finally, class V corresponds to intermediate filaments of the nuclear cytoskeleton, the nuclear lamins. Neurofibrils are bundles of neurofilaments.

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2419

Applications

WB; IHC-P

Application Notes

WB (1:500-1:1000); IHC (1:500-1:1000)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA.

Concentration

Batch Dependent

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

NEFH

Official Name

NEFH

Full Name

Hypophosphorylated Neurofilament H

Alternative Names

Neurofilament Heavy; Neurofilament Heavy Polypeptide; Neurofilament; Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; 200 KDa Neurofilament Protein; NF-H; NFH; KIAA085; CMT2CC;