NeuroMab™ Mouse Anti-CNPase Monoclonal Antibody (CBP2444)

Description

High CNPase expression is seen in myelin producing cells, including oligodendrocytes and Schwann cells. CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (CNS). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth. The enzyme isolated from the mammalian brain is primarily a mixed dimer of approximately 94 kD. The dimer consists of a varied proportion of CNP1 (46 kD) and CNP2 (48 kD) subunits in various species. Since the enzyme is a myelin-associated enzyme, it is of considerable interest in the study of diseases and disorders in which myelin is affected, such as multiple sclerosis, subacute sclerosing panencephalitis, acquired immunodeficiency with CNS involvement, and peripheral neuropathies. The combination of clone SMI 91 with clone SMI 94 and/or clone SMI 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2444

Applications

IHC-P; WB; IHC-Fr; ICC; IP

Application Notes

IHC-P (1.0 µg/mL); WB (0.5-5.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1788

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

Batch Dependent

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Myelin CNPase

Official Name

CNP

Full Name

CNPase

Alternative Names

CNP; CNP1; 2'; 3'-cyclic nucleotide 3' phosphodiesterase; CNPase

Gene ID

1267 (Human); 12799 (Mouse); 25275 (Rat)

Uniprot ID

P09543 (Human); P16330 (Mouse); P13233 (Rat)

1. Han H, et al. 2013. Biofactors. 39(3):233.
2. Raasakka A, et al. 2014. Neurosci Bull. 30(6):956.
3. Kim JY, et al. 2003. J. Neurosci. 23(13):5561-5571.
4. Sprinkle TJ, et al. 1989. Crit. Rev. Neurobiol. 4:235-301.
5. Ding BS, et al. 2013. PLoS One 8:e62150.
6. Ferletta M, et al. 2011. Int. J. Cancer 129:45.