NeuroMab™ Rat Anti-Mouse Vimentin Monoclonal Antibody (CBP2466)

Product Overview

Description

Vimentin are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is a widely expressed and highly conserved 54 kD protein that is constitutively expressed in mesenchymal cells, endothelial cells lining blood vessels, renal tubular cells, macrophages, neutrophils, fibroblasts, and leukocytes1,2. Vimentin is used as a marker of mesenchymal cells to distinguish them from epithelial cells3. Increased vimentin expression is frequently used as an EMT marker in cancer4. Autoantibodies to vimentin are commonly found in patients with autoimmune diseases such as Lupus5 and rheumatoid arthritis6, and also found after transplantation7.

Immunogen

Partial mouse Vimentin (224-446 a.a.) recombinant protein expressed in E. coli.

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2a, κ

Clone Number

CBP2466

Applications

WB; ICC

Application Notes

WB (0.1- 1 µg/mL); ICC (0.2- 2.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1805

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

Batch Dependent

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Vimentin

Official Name

VIM

Full Name

Vimentin

Alternative Names

VIM; CTRCT30; HEL113; vimentin

Gene ID

7431(Human); 22352(Mouse); 81818(Rat)

Uniprot ID

P08670(Human); P20152(Mouse); P31000(Rat)

References

1. Kidd ME, et al. 2014. Am J Respir Cell Mol Biol. 50:1.
2. Fuchs E, et al. 1994. Annu Rev Biochem. 63:345.
3. Zeisberg M, et al. 2009. J Clin Invest. 119:1429.
4. Scanlon CS, et al. 2013. J Dent Res. 92:114.
5. Thebault S, et al. 2002. J Immunol. 169:4046.
6. Vossenaar ER, et al. 2004. Arthritis Res Ther. 6:R142.
7. Rose ML. 2013. Hum Immunol. 74:1459.

Publications