Rat Anti-IL-10 Monoclonal Antibody (CBP2532)

Product Overview

Description

The rat anti-Mouse Interleukin-10 antibody, recognizes mouse inhibitor of cytokine synthesis (CSIF), also known as interleukin 10 (IL-10). IL-10 is produced by B cells expressing Ly-1 (CD5) (O'Garra et al., 1992) and a portion of stimulated T cells (Asseman et al., 1999). IL-10 has a wide range of biological properties, including inhibition of cytokine synthesis mediated by macrophages and T helper cells, and inhibition of delayed-type hypersensitivity. According to reports, rat anti-mouse IL-10 antibody clone JES5-2A5 can neutralize the activity of murine IL-10.

Immunogen

Recombinant mouse IL-10.

Species Reactivity

Mouse

Clonality

monoclonal

Host Species

Rat

Isotype

IgG1

Clone Number

CBP2532

Applications

ELISA; FC; IHC-Fr; WB

Application Notes

ELISA: 1/200-1/2000
WB: 1/1000
FC: 1/10-1/100

Relevant Diseases

Amyotrophic Lateral Sclerosis; Frontotemporal Dementia

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.09% Sodium Azide

Preservatives

YES

Concentration

1 mg/ml

Purification

Protein G

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

IL-10

Official Name

IL10

Full Name

Interleukin 10

Alternative Names

Interleukin 10

Gene ID

3586(Human)

Uniprot ID

P22301(Human)

References

1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
2. Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
3. Nishigori, C. et al. (1998) HindIII liposomes suppress delayed-type hypersensitivity responses in vivo and induce epidermal IL-10 in vitro.

Publications