Mouse Anti-FOS Monoclonal Antibody (CBP2809)

Product Overview

Description

c-FOS contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. FOS is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, FOS undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the FOS protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the FOS protein becomes nuclear. The calculated molecular weight of FOS is 40 kDa, but Phosphorylated FOS protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development.

Expression Host

Mammalian cells

Immunogen

FOS fusion protein Ag24340

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2809

Applications

IHC; WB; ELISA

Application Notes

IHC : 1:20-1:200; WB : 1:500-1:1000

Research Areas

Metabolism

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1000 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

c-Fos

Official Name

FOS

Full Name

FOS

Alternative Names

AP 1; C FOS; Cellular oncogene fos; C-FOS; FOS; G0S7; Proto oncogene c Fos

Gene ID

2353(Human); 14281(Mouse)

Uniprot ID

P01100(Human); P01101(Mouse)

References

1. Zhang, Y., Gao, J., Sun, W., Wen, X., Xi, Y., Wang, Y.,... & Li, H. (2019). H2S restores the cardioprotective effects of ischemic post-conditioning by upregulating HB-EGF/EGFR signaling. Aging (Albany NY), 11(6), 1745. 2. Li, M., Bian, Z., Jin, G., Zhang, J., Yao, S., Feng, Y.,... & Huang, Z. (2019). Lnc RNA-SNHG 15 enhances cell proliferation in colorectal cancer by inhibiting miR-338-3p. Cancer medicine, 8(5), 2404-2413. 3. Li, Q., Wang, T., Huang, S., Zuo, Q., Jiang, Z., Yang, N., & Sun, L. (2020). LncRNA MALAT1 Affects the Migration and Invasion of Trophoblast Cells by Regulating FOS Expression in early-onset Preeclampsia. Pregnancy Hypertension.

Publications