Mouse Anti-PPARG Monoclonal Antibody (CBP2873)

Description

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, INS resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 INS-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887).

Expression Host

Mammalian cells

Immunogen

PPARG fusion protein Ag16657

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2873

Applications

IF; IHC; WB; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; WB : 1:500-1:1000

Research Areas

Metabolism

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1000 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

PPAR Gamma

Official Name

PPARG

Full Name

Peroxisome proliferator-activated receptor gamma

Alternative Names

CIMT1; NR1C3; PPAR gamma; PPARG; PPARG1

Gene ID

5468(Human)

Uniprot ID

P37231(Human)

1. Chen, C., Deng, Y., Hu, X., Ren, H., Zhu, J., Fu, S.,... & Peng, Y. (2018). miR-128-3p regulates 3T3-L1 adipogenesis and lipolysis by targeting Pparg and Sertad2. Journal of physiology and biochemistry, 74(3), 381-393.