Mouse Anti-Human ASC Monoclonal Antibody (CBP3987)

Description

Apoptosis-related punctate protein (CSC) (ASC), also known as methylation-mediated silencing (TMS1), is the target of PYD and CARD domain-containing protein (PYCARD), and is a 21.6 kD pro-apoptosis Death protein, which contains the N-terminal pyridine domain (PYD) and the C-terminal caspase recruitment domain (CARD). It was initially found that the TMS1 gene was abnormally methylated and silenced in various cancer cells. The expression of ASC can be induced by pro-apoptotic/inflammatory stimuli. In normal cells, proteins are located in the cytoplasm. However, in cells undergoing apoptosis, it forms spherical aggregates near the periphery of the nucleus. In the process of apoptosis, ASC transfers from the cytosol to the mitochondria and binds to the mitochondria Bax, which triggers the release of cytochrome c and subsequent apoptosis. The adaptor molecule ASC mediates AIM2-dependent caspase-1 activation. In addition, the AIM2/ASC complex can act as a new type of caspase-8 activation platform. Recent studies have shown that ASC reduces the phosphorylation of IKKα/β phosphorylation and inhibits the activity of NF-κB in primary melanoma. However, in metastatic melanoma, it has the opposite function of enhancing NF-κB activity and IL-1β secretion.

Immunogen

Full length recombinant human PYCARD (NP_037390) produced in E. coli.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP3987

Applications

WB

Application Notes

WB (0.5-2.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

1.0 mg/mL

Purification

Affinity Chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

ASC

Official Name

PYCARD

Full Name

Apoptosis-associated speck-like protein

Alternative Names

PYCARD; ASC; CARD5; TMS; TMS-1; TMS1; PYD and CARD domain containing

Gene ID

29108 (Human); 66824 (Mouse)

Uniprot ID

Q9ULZ3 (Human); Q9EPB4 (Mouse)

1. Masumoto J, et al. 1999. J. Biol. Chem. 274:33835.
2. Conway KE, et al. 2000. Cancer Res. 60:6236.
3. Strong R, et al. 1991. Brain Res. 542:23.
4. Ohtsuka T, et al. 2004. Nat. Cell Biol. 6:121.
5. Srinivasula SM, et al. 2002. J. Biol. Chem. 277:21119.
6. Ippagunta SK, et al. 2010. J. Biol. Chem. 285:12454.