Anti-CD178 Monoclonal Antibody (CBP4011)

Product Overview

Description

CD178 is a 38-42 kD type II glycoprotein also known as Fas ligand and CD95L. CD178 belongs to the TNF superfamily and is expressed on activated T lymphocytes, NK cells, monocytes, and granulocytes. CD178 is also expressed on parenchymal cells of the retina and cornea, retinal pigment epithelial cells, and testis. The extracellular region of FasL can be cleaved by matrix metalloproteinases (MMPs) to give rise to a 26 kD soluble protein. CD178 binds to CD95, a member of the TNFR superfamily, to induce apoptosis. CD95/CD95L interactions play an important role in the maintenance of peripheral tolerance and survival.

Immunogen

B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells

Species Reactivity

Mouse; Rat

Clonality

Monoclonal

Host Species

Armenian Hamster

Isotype

IgG

Clone Number

CBP4011

Applications

FC; Block

Application Notes

FCM (≤ 0.25 µg per million cells in 100 µL volume)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

0.2 µm filtered in PBS, pH 7.2, containing no preservative.

Concentration

1.0 mg/mL

Purification

Affinity Chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD178

Official Name

FASLG

Full Name

Fas ligand

Alternative Names

FASLG; ALPS1B; APT1LG1; APTL; CD178; CD95-L; CD95L; FASL; TNFSF6; TNLG1A; Fas ligand

Gene ID

356 (Human); 14103 (Mouse)

Uniprot ID

P48023 (Human); P41047 (Mouse)

References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Nagata S. 1999. Annu. Rev. Genet. 33:209.
3. Takahashi T, et al. 1994. Cell 76:969.
4. Hill LL, et al. 1999. Science 285:898.

Publications