Rat Anti-Mouse LRP2 Monoclonal Antibody (CBP4241)

Description

Megalin or low density lipoprotein-related protein 2 (LRP2), is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithelial tissues such as the kidney. LRP2 mediates uptake of Apo-J which is a binding protein for Amyloid beta. Megalin has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. Megalin is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. It also has a role in cell-signaling; extracellular ligands include parathyroid hormones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in the LRP2 gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).

Immunogen

This monoclonal antibody was raised against purified human LRP2 protein.

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG1

Clone Number

CBP4241

Applications

IHC-P; IHC-F

Application Notes

IHC ( 5.0-10 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

1 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

LRP2

Official Name

LRP2

Full Name

Low density lipoprotein-related protein 2

Alternative Names

LRP2; Low density lipoprotein-related protein 2

Gene ID

4036(Human); 14725(Mouse); 29216(Rat)

Uniprot ID

P98164(Human); A2ARV4(Mouse); P98158(Rat)