Mouse Anti-Neurofilament Light chain, NF-L. Monoclonal Antibody (CBP6631)

Description

Neurofilaments are 10nm or intermediate filament proteins that are specifically found in neurons. They are mainly composed of three main proteins called NF-L, NF-M and NF-H, but they may also include other filament proteins. The main function of nerve filaments may be to control the diameter of large axons (1). NF-L is a neurofilament light or low molecular weight peptide. It runs on a 68-70kDa SDS-PAGE gel. There are some differences between different species. Antibodies against NF-L (such as MCA-7D1) can be used to identify neuronal cells and their processes in cell culture and sectioning materials. NF-L antibody can also be used to visualize the accumulation of neurofilaments that is seen in many neurological diseases, such as Lou Gehrig's disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease, etc. (2-4). Recently, a lot of attention has been paid to the detection of NF-L released from neurons into the blood and cerebrospinal fluid as a surrogate marker of major axonal loss, which is the main cause of various types of central nervous system damage and degeneration (5 ).

Immunogen

Full-length natural protein purified from porcine spinal cord

Species Reactivity

Human; Rat; Mouse; Cow; Porcine; Horse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b

Clone Number

CBP6631

Applications

WB; IF; IHC

Application Notes

WB: 1:5000-1:10000
IF/ICC and IHC: 1:100-1:500

Form

Liquid

Formulation

50% PBS, 50% glycerol

Preservatives

5mM NaN3

Concentration

1 mg/ml

Shipping

Gel Pack

Storage

Store at 4°C for short term, for longer term at -20°C

Handling Advice

The antibody solution should be gently mixed before use.

Research Use Only

For research use only

Target

Neurofilament L

Official Name

NEFL

Full Name

Neurofilament light polypeptide

Alternative Names

NEFL; CMT1F; CMT2E; NF-L; NF68; NFL; PPP1R110; neurofilament; light polypeptide; neurofilament light; CMTDIG

Gene ID

4747 (Human); 18039 (Mouse); 83613 (Rat)

Uniprot ID

P07196 (Human); P08551 (Mouse); P19527 (Rat)