Mouse Anti-Ankyrin 3 Monoclonal Antibody (CBP6678)

Description

The first ankyrin was isolated from the red blood cell membrane and was found to be responsible for anchoring the spectrin cytoskeleton to the plasma membrane. Subsequently, this protein was named Ankyrin 1, and a close homologue was named Ankyrin 2, although these two proteins are also known as Ankyrin R and Ankyrin B, which are used in red blood cells and the brain, respectively (2 ). Both proteins are large, about 200kDa. The third member of the protein family was discovered and called Ankyrin 3, also known as Ankyrin G, some of which have a much larger molecular size, up to 480kDa. G refers to giant or general, because this protein is large and widely expressed (2). All three ankyrins have N-terminal fragments composed of 22 tandem repeats, each of which consists of 33 amino acids. These sequences are called ankyrin-type repeats. A subset of these repetitive sequences is responsible for binding ankyrin proteins to various membrane proteins, and such repetitive sequences are found in many other proteins and usually mediate specific protein-protein interactions (3).

Immunogen

The C-terminal 398 amino acids of human ankyrin 3 expressed and purified in E. coli

Species Reactivity

Human; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP6678

Applications

WB; IF; IHC

Application Notes

WB: 1:1000-1:2000
ICC/IF and IHC: 1:1000

Form

Liquid

Formulation

50% PBS, 50% glycerol

Preservatives

5mM NaN3

Concentration

1 mg/ml

Shipping

Gel Pack

Storage

Store at 4°C for short term, for longer term at -20°C

Handling Advice

The antibody solution should be gently mixed before use.

Research Use Only

For research use only

Target

ANK-3

Official Name

ANK3

Full Name

Ankyrin-3

Alternative Names

ANK3; ANKYRIN-G; MRT37; ankyrin 3; node of Ranvier (ankyrin G); ankyrin 3

Gene ID

288 (Human); 11735 (Mouse); 361833 (Rat)

Uniprot ID

Q12955 (Human); G5E8K5 (Mouse); O70511 (Rat)