Rat Syndecan-1/CD138(SDC1) ELISA Kit

Name: Rat Syndecan-1/CD138(SDC1) ELISA Kit
SKU: NRZP-0722-ZP232
Rating: 5 (1 reviews)

[CAT#: NRZP-0722-ZP232]

For the quantitative determination of rat syndecan-1/CD138 (SDC1) concentrations in serum, plasma, tissue homogenates.

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of rat SDC1.

Species Reactivity

Rat

Marker

Syndecan-1/CD138(SDC1)

Properites

Sensitivity

In most cases, the sensitivity is below 0.078 ng/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, tissue homogenates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

0.312 ng/mL-20 ng/mL

Max Emission

450 nm

Target Details

Target

CD138 / Syndecan 1

Official Name

SDC1

Full Name

syndecan 1

Alternative Names

CD138, SDC, SYND1, syndecan, CD138 antigen, heparan sulfate proteoglycan fibroblast growth factor receptor, syndecan proteoglycan 1

Gene ID

25216(Rat)

Uniprot ID

P26260(Rat)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

Publications