Thioredoxin Reductase Activity Colorimetric Assay Kit, Mammalian

Description

Thioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme that participates in many cellular processes, such as cell growth, p53 activity, and anti-oxidative stress. Mammalian TrxR can reduce thioredoxin and non-disulfide substrates. For example selenite, lipoic acid, lipid hydroperoxide and hydrogen peroxide. Our thioredoxin reductase assay kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the determination, TrxR catalyzes NADPH to reduce 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) to 5-thio-2-nitrobenzoic acid (TNB2-), resulting in a strong yellow (λmax= 412 nm)). Since other enzymes (such as glutathione reductase and glutathione peroxidase) can also reduce DTNB in ​​the original biological sample, specific inhibitors of TrxR can be used to determine the specific activity of TrxR. Two determinations are performed: the first determination is the total reduction of DTNB in ​​the sample, and the second is the reduction in the amount of DTNB in ​​the sample in the presence of a TrxR specific inhibitor. The difference between the two results is that TrxR reduces DTNB.

Species Reactivity

Mammalian

Relevant Diseases

Neurological Disorders

Detection Method

Absorbance (412 nm)

Components

• TrxR Assay Buffer
• TNB Standard
• DTNB
• NADPH
• TrxR Positive Control
• TrxR Inhibitor

Shipping

Gel Pack

Storage

-20°C

Research Use Only

For research use only

Target

Thioredoxin

Official Name

TXN

Full Name

Thioredoxin

Alternative Names

Thioredoxin; Surface-Associated Sulphydryl Protein; ATL-Derived Factor; SASP; TRX1; TRDX

Gene ID

7295(Human); 22166(Mouse)

Uniprot ID

P10599(Human); P10639(Mouse)