AKT 1/2/3 pS473 / AKT1 ELISA Kit [Colorimetric]

[CAT#: NPP2011ZP285]

Applications:: ELISA

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Product Overview

Description

AKT 1/2/3 (pS473) and AKT1 (Total) in vitro SimpleStep™ ELISA (enzyme-linked immunosorbent assay) kits are used to detect AKT 1/2/3 (pS473) and total in human and mouse cells. AKT1 protein was measured semi-quantitatively.

Species Reactivity

Human

Applications

ELISA

Pathways

Kinase

Properites

Sensitivity

30 pg/ml

Platform

Microplate

Detection Method

Colorimetric

Sample Type

Cell Lysate, Tissue Homogenate

Assay Type

Semi-quantitative

Assay Time

1h 30m

Assay Duration

One step assay

Components

Components 1 x 96 tests
10X Wash Buffer PT 1 x 15ml
50X Cell Extraction Enhancer Solution 1 x 1ml
5X Cell Extraction Buffer PTR 1 x 12ml
AKT 1/2/3 (pS473) Capture Antibody 1 x 1.5ml
AKT 1/2/3 (pS473) Detector Antibody 1 x 1.5ml
AKT1 (Total) Capture Antibody 1 x 1.5ml
AKT1 (Total) Detector Antibody 1 x 1.5ml
Lyophilized AKT Control Lysate 1 vial
Plate Seal 1 unit
SimpleStep Pre-Coated 96-Well Microplate 1 unit
Stop Solution 1 x 12ml
TMB Substrate 1 x 12ml

Preparation and Storage

Storage

Store at +4°C. Please refer to protocols.

Research Use Only

For research use only

Target Details

Target

AKT1

Official Name

AKT1

Full Name

v-akt murine thymoma viral oncogene homolog 1

Alternative Names

Protein kinase B alpha; Proto-oncogene c-Akt; RAC; PKB alpha; RAC-ALPHA; CWS6; PRKBA; AKT; PKB; RAC-PK-alpha; PKB-ALPHA; RAC-alpha serine/threonine-protein kinase; EC 2.7.11.1; Protein kinase B