Product
AKT1 / AKT2 / AKT3 (pS473) ELISA Kit [Colorimetric], Semi-quantitative
[CAT#: NPP2011ZP287]
- Applications:
- ELISA
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This is a very fast, convenient and sensitive detection kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By measuring the phosphorylated Akt protein in your experimental model system, you can verify the pathway activation in your cell lysate. You can measure many different cell lysates at the same time without spending extra time and effort for Western Blotting analysis.
Species Reactivity
Human
Applications
ELISA
Pathways
Kinase
Properites
Platform
Microplate
Detection Method
Colorimetric
Sample Type
Cell Lysate
Assay Type
Semi-quantitative
Assay Time
5h 00m
Assay Duration
Multiple steps standard assay
Components
Components 1 x 96 tests
20X Wash Buffer 1 x 25ml
2X Cell Lysis Buffer 1 x 5ml
5X Assay Diluent 1 x 15ml
Akt Microplate (12 strips x 8 wells) coated with anti-pan Akt antibody 1 unit
Detection Antibody Akt (Ser473): rabbit anti-phospho-Akt (Ser473) 2 vials
500X HRP-conjugated anti-rabbit IgG 1 x 25µl
Positive Control: lyophilized NIH3T3 cell lysate 1 vial
Stop Solution 1 x 8ml
TMB One-Step Substrate Reagent 1 x 12ml
20X Wash Buffer 1 x 25ml
2X Cell Lysis Buffer 1 x 5ml
5X Assay Diluent 1 x 15ml
Akt Microplate (12 strips x 8 wells) coated with anti-pan Akt antibody 1 unit
Detection Antibody Akt (Ser473): rabbit anti-phospho-Akt (Ser473) 2 vials
500X HRP-conjugated anti-rabbit IgG 1 x 25µl
Positive Control: lyophilized NIH3T3 cell lysate 1 vial
Stop Solution 1 x 8ml
TMB One-Step Substrate Reagent 1 x 12ml
Preparation and Storage
Storage
Store at +4°C. Please refer to protocols.
Research Use Only
For research use only
Target Details
Target
AKT1
Official Name
AKT1
Full Name
v-akt murine thymoma viral oncogene homolog 1
Alternative Names
Protein kinase B alpha; Proto-oncogene c-Akt; RAC; PKB alpha; RAC-ALPHA; CWS6; PRKBA; AKT; PKB; RAC-PK-alpha; PKB-ALPHA; RAC-alpha serine/threonine-protein kinase; EC 2.7.11.1; Protein kinase B
Publications
Publications (0)
Neural Antibodies
Kits
For Research Use Only. Not For Clinical Use.
