Brain Tumor Dissociation Kit (P), Human and Mouse

[CAT#: NRZP-0622-ZP17]

Applications:: Tissue Dissociation

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Product Overview

Description

The Brain Tumor Isolation Kit (P) was developed for gentle and efficient tissue isolation of human and mouse brain tumors. Glioblastoma, neuroblastoma, medulloblastoma, xenograft brain tumor samples can be efficiently isolated into single cell suspensions using the Brain Tumor Isolation Kit (P). Brain Tumor Isolation Kit (P) is based on papain. In less than an hour, the tissue dissociation process effectively yields large numbers of living cells. Dissociated cells can be used immediately for further applications such as cell culture, flow cytometry, molecular applications.

Applications

Tissue Dissociation

Application Notes

When working with immune cells from human, mouse, or xenograft tumor, it is recommend to use in this case the Tumor Dissociation Kit, mouse (# NRZP-0622-ZP18) or Tumor Dissociation Kit, human (# NRZP-0622-ZP19) depending on the species from where the tumor originated.

Relevant Diseases

Tumor

Principle

Brain tumor tissue can be dissociated into single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular matrix. Enzymatic digestion of tumor tissue using kit components. After dissociation, the sample is applied to a filter to remove any remaining larger particles from the single-cell suspension. Alternatively, Myelin Removal Beads II can be used to remove myelin to improve results in downstream applications such as molecular and flow cytometry analysis or cell isolation.