Creative Biolabs

Cell Quantifying Kit

[CAT#: NCC200556ZP]

The Cell Quantifying Kit uses WST-8 to conveniently determine the number of viable cells in cell proliferation and cytotoxicity assays.

Applications:
Cell Culture
Cell Types:
Neural Lineage Cell

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Description
The Cell Quantitation Kit uses WST-8 to easily determine the number of viable cells in cell proliferation and cytotoxicity assays. The reagent will be reduced due to the metabolic activity of living cells, and the amount of formazan produced is proportional to the number of living cells. One bottle of Kit can be tested 500 times without pre-mixing. Cell proliferation assays using Kit have been shown to be closely related to [3H]-thymidine incorporation assays. In addition, compared with other tetrazolium salts (such as MTT, XTT, MTS or WST-1), the kit shows a higher detection sensitivity.

Features
High sensitivity
No cytotoxicity
Long shelf life
Simple steps
Cell Types
Neural Lineage Cell
Species Reactivity
Human; Mouse; Rat
Applications
Cell Culture
Research Areas
Stem Cell Research
Shipping
Room Temperature
Storage
Store at room temperature (stable for up to 6 months), 0-5°C (stable for up to 1 year) or -20°C (stable for up to 2 years).
For frequent use, please store at 0-5°C. Repeated thawing and freezing will cause an increase in background and thus interfere with the determination.
Handling Advices
Cell Counting Protocol
Inoculate the cell suspension (100μl/well) into a 96-well plate. Pre-incubate the plate in a humidified incubator (for example at 37°C, 5% CO2).
Add 10μl kit solution to each well of the plate.
Be careful not to introduce air bubbles into the hole, because they will affect the outer diameter. read.
 Incubate the plate for 1-4 hours in an incubator.
Measure the absorbance at 450 nm with a microplate reader.
To measure the absorbance later, add 10 μl of 1% w/v SDS or 0.1 M HCl to each well, cover the plate and store at room temperature protected from light. No change in absorbance was observed within 24 hours.

Cell proliferation/cytotoxicity Protocol
 Dispense 100 μl of cell suspension (5000 cells/well) in a 96-well plate. Pre-incubate the plate in a humid incubator for 24 hours (for example, at 37°C, 5% CO2).
Add 10μl of test substance with various concentrations on the plate.
 Incubate the plate in the incubator for an appropriate time (for example, 6, 12, 24, or 48 hours).
Add 10μl kit solution to each well of the plate.
Be careful not to introduce air bubbles into the hole, because they will affect the outer diameter. read.
 Incubate the plate for 1-4 hours in an incubator.
Measure the absorbance at 450 nm with a microplate reader.
To measure absorbance later, add 10 μl of 1% w/v SDS or 0.1 M HCl to each well, cover the plate, and store at room temperature protected from light. No change in absorbance was observed within 24 hours.
Quality Control
Each batch is tested for appearance, blank and sensitivity.
Shelf Life
Stable up to 6 months
Research Use Only
For research use only, not for diagnostic or therapeutic use.

For Research Use Only. Not For Clinical Use.
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