
Cell Quantifying Kit
The Cell Quantifying Kit uses WST-8 to conveniently determine the number of viable cells in cell proliferation and cytotoxicity assays.
- Applications:
- Cell Culture
- Cell Types:
- Neural Lineage Cell
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
Features
High sensitivity
No cytotoxicity
Long shelf life
Simple steps
Cell Types
Species Reactivity
Applications
Research Areas
Shipping
Storage
For frequent use, please store at 0-5°C. Repeated thawing and freezing will cause an increase in background and thus interfere with the determination.
Handling Advices
Inoculate the cell suspension (100μl/well) into a 96-well plate. Pre-incubate the plate in a humidified incubator (for example at 37°C, 5% CO2).
Add 10μl kit solution to each well of the plate.
Be careful not to introduce air bubbles into the hole, because they will affect the outer diameter. read.
Incubate the plate for 1-4 hours in an incubator.
Measure the absorbance at 450 nm with a microplate reader.
To measure the absorbance later, add 10 μl of 1% w/v SDS or 0.1 M HCl to each well, cover the plate and store at room temperature protected from light. No change in absorbance was observed within 24 hours.
Cell proliferation/cytotoxicity Protocol
Dispense 100 μl of cell suspension (5000 cells/well) in a 96-well plate. Pre-incubate the plate in a humid incubator for 24 hours (for example, at 37°C, 5% CO2).
Add 10μl of test substance with various concentrations on the plate.
Incubate the plate in the incubator for an appropriate time (for example, 6, 12, 24, or 48 hours).
Add 10μl kit solution to each well of the plate.
Be careful not to introduce air bubbles into the hole, because they will affect the outer diameter. read.
Incubate the plate for 1-4 hours in an incubator.
Measure the absorbance at 450 nm with a microplate reader.
To measure absorbance later, add 10 μl of 1% w/v SDS or 0.1 M HCl to each well, cover the plate, and store at room temperature protected from light. No change in absorbance was observed within 24 hours.