CRIGEM™ iPSC-Derived Neural Stem Cells, MAPT V337M HOM
Please be aware that the correct volume of Neural Plating Medium (Cat. NCC-2101-ZP07) (not Xeno-Free) needed for your specific order volume of cells will be supplied FREE OF CHARGE.
The V337M mutation in MAPT has been implicated in frontotemporal dementia and may have a role in Alzheimer's disease. The mutation leads to accelerated aggregation of MAPT/tau protein into filament structures (Nacharaju et al., 1999) and makes the MAPT/tau protein more susceptible to hyperphosphorylation (Alonso et al., 2004).
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.