Creative Biolabs

CRIGEM™ iPSC-Derived Neural Stem Cells, MAPT V337M HOM

[CAT#: NCL-2101-ZP17]

Please be aware that the correct volume of Neural Plating Medium (Cat. NCC-2101-ZP07) (not Xeno-Free) needed for your specific order volume of cells will be supplied FREE OF CHARGE.

Species:
Human
Applications:
Cell Assay
Cell Types:
Neural Stem Cell

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Description
Human iPSC-Derived Neural Stem Cells that have been genetically edited using CRISPR-Cas9 technology to introduce the V337M mutation (GTG>ATG) in the MAPT gene. This line is homozygous for the V337M mutation so both alleles contain the mutation. Click on the product images to see the data and further details.

The V337M mutation in MAPT has been implicated in frontotemporal dementia and may have a role in Alzheimer's disease. The mutation leads to accelerated aggregation of MAPT/tau protein into filament structures (Nacharaju et al., 1999) and makes the MAPT/tau protein more susceptible to hyperphosphorylation (Alonso et al., 2004).
Cell Types
Neural Stem Cell
Applications
Cell Assay
Species
Human
Size
1.5 million cells & Neural Plating-XF Medium
Growth Pattern
Adherent
Tissue Source
Fibroblasts (64 Yr Donor)
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.
For Research Use Only. Not For Clinical Use.
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