Estradiol, Rat

Description

Estradiol (E2 or 17β-estradiol) is the main female reproductive hormone secreted by the ovaries. The adrenal cortex and testes also produce small amounts of estradiol. Our rat estradiol E2 ELISA kit is based on sandwich enzyme-linked immunosorbent In the experiment, the rat estradiol E2 antibody was coated on a 96-well plate, the standard and test samples were added to the wells, the estradiol E2 present in the sample was combined with the immobilized antibody, and the HRP coupling reagent was added to wash it away After the unbound antibody/HRP conjugate, the HRP substrate TMB is used to observe the enzymatic reaction of HRP. HMB catalyzes TMB to produce a blue product, which turns yellow after adding acidic stop solution. The yellow density is the same as that of the rat captured on the plate. Estradiol E2 is directly proportional, and the ELISA kit has no species cross-reactivity detection range: 2 - 50 ng/L.

This ELISA kit is used for quantitative detection of Estradiol, establishing normal range.

Species Reactivity

Rat

Relevant Diseases

Neurological Disorders

Detection Method

Absorbance (450 nm)

Assay Type

ELISA kit

Detection Range

2 - 50 ng/L

Components

• 96 wells coated with anti-rat Estradiol antibody, 1 Microplate with 2 adhesive strips
• Rat Estradiol standard (72 pmol/L)
• HRP-conjugate reagent
• Standard Diluent
• Sample diluent
• Chromogen Solution A
• Chromogen Solution B
• Stop Solution
• Wash Solution (30x stock)

Shipping

Gel Pack

Storage

4°C

Research Use Only

For research use only

Target

Estradiol

Official Name

E2

Full Name

Estradiol

Alternative Names

Oestradiol; E2; 17β-Estradiol; Estra-1; 3; 5(10)-triene-3; 17β-diol; 17β-Oestradiol