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Creative Biolabs
Product

Human FMR1 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP254]

Human FMR1 knockout cell lysate

Species:
Human
Cell Types:
HEK293T

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human FMR1 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HEK293T

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and Insertion of the selection cassette in exon 4.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neural Cell

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

FMR1

Official Name

FMR1

Full Name

fragile X mental retardation 1

Alternative Names

FMR1; FMRP; FRAXA; POF; POF1; fragile X mental retardation 1; FMRP translational regulator 1

Gene ID

2332(Human)

Uniprot ID

Q06787(Human)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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