Human nuclear factor-κB p65, NF-κB p65 ELISA Kit

[CAT#: NRZP-0722-ZP254]

For the quantitative determination of human nuclear factor-κB p65 (NF-κB p65) concentrations in serum, plasma, tissue homogenates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human NF-κB p65.

Species Reactivity

Human

Marker

V-rel reticuloendotheliosis viral oncogene homolog A (avian)

Properites

Sensitivity

In most cases, the sensitivity is below 11.75 pg/ml.

Detection Method

Sandwich

Sample Type

serum, plasma and tissue homogenates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

47 pg/ml-3000 pg/ml

Max Emission

450 nm

Target Details

Target

RELA

Official Name

RELA

Full Name

RELA proto-oncogene, NF-kB subunit

Alternative Names

p65; CMCU; NFKB3

Gene ID

5970(Human)

Uniprot ID

Q04206(Human)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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