Creative Biolabs

Human Phospho-NF-kappaB p65 (S468 / S536) / Total In-Cell ELISA Kit [Colorimetric]

[CAT#: NPP2011ZP455]

Applications:
ELISA; In-Cell ELISA

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Description
The phosphp-nf-kappab p65 (S468 + S536) + Total In-Cell ELISA kit provides a simple, efficient, cell-based method for the detection of phosphorylated activated protein. This kit is specifically designed to quantify activated (phosphorylated) NF-kappaB p65 and/or total NF-kappaB p65. Cells are cultured in 96-well plates and stimulated to induce pathways of interest. After stimulation, cells are quickly fixed to preserve activation-specific protein modifications. Each well is then incubated with a primary antibody that can recognize phosphorylated NF-kappaB p65 or total NF-kappaB p65. Subsequent incubation with hrp-labeled secondary antibody and developing solution provides an easily quantifiable colorimetric reading. Then use the provided crystal violet solution to determine the relative number of cells per well. The 96-well plate format is suitable for high-throughput screening applications.
Species Reactivity
Human
Applications
ELISA; In-Cell ELISA
Research Areas
Neurodegenerative Diseases
Pathways
Alzheimer's Disease Signaling
Marker
Tangles & Tau
Platform
Microplate
Detection Method
Colorimetric
Sample Type
Adherent cells, Suspension cells
Assay Type
Cell-based (quantitative)
Assay Duration
Multiple steps standard assay
Components
Components 3 x 96 tests
1% SDS Solution 2 x 22ml
10% Triton X-100 1 x 10ml
10X PBS 2 x 120ml
1X Antibody Blocking Buffer 2 x 22ml
1X Antibody Dilution Buffer 2 x 30ml
96-well tissue culture plate 3 units
Anti-rabbit HRP-conjugated IgG 2 x 11µl
Crystal Violet Solution 2 x 22ml
Developing Solution 3 x 11ml
Phospho-NFkB p65 (Ser468) antibody 1 x 9µl
Phospho-NFkB p65 (Ser536) antibody 1 x 9µl
Plate sealer 3 units
Stop Solution 3 x 11ml
Total-NFkB antibody 1 x 9µl
Storage
Store at +4°C. Please refer to protocols.
Research Use Only
For research use only
Target
NFKB1
Official Name
NFKB1
Full Name
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Alternative Names
NF-kB1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkappaB; p105; EBP-1; NF-kappa-B; NF-kappaB; NFKB-p50; KBF1; DNA-binding factor KBF1; p50; NFKB-p105
Gene ID
4790(Human);18033(Mouse)
Uniprot ID
P19838(Human);P25799(Mouse)
For Research Use Only. Not For Clinical Use.
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