Human Phospho-NF-kappaB p65 (S468 / S536) / Total In-Cell ELISA Kit [Colorimetric]

[CAT#: NPP2011ZP455]

Applications:: ELISA; In-Cell ELISA

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Product Overview

Description

The phosphp-nf-kappab p65 (S468 + S536) + Total In-Cell ELISA kit provides a simple, efficient, cell-based method for the detection of phosphorylated activated protein. This kit is specifically designed to quantify activated (phosphorylated) NF-kappaB p65 and/or total NF-kappaB p65. Cells are cultured in 96-well plates and stimulated to induce pathways of interest. After stimulation, cells are quickly fixed to preserve activation-specific protein modifications. Each well is then incubated with a primary antibody that can recognize phosphorylated NF-kappaB p65 or total NF-kappaB p65. Subsequent incubation with hrp-labeled secondary antibody and developing solution provides an easily quantifiable colorimetric reading. Then use the provided crystal violet solution to determine the relative number of cells per well. The 96-well plate format is suitable for high-throughput screening applications.

Species Reactivity

Human

Applications

ELISA; In-Cell ELISA

Research Areas

Neurodegenerative Diseases

Pathways

Alzheimer's Disease Signaling

Marker

Tangles & Tau

Properites

Platform

Microplate

Detection Method

Colorimetric

Sample Type

Adherent cells, Suspension cells

Assay Type

Cell-based (quantitative)

Assay Duration

Multiple steps standard assay

Preparation and Storage

Storage

Store at +4°C. Please refer to protocols.

Research Use Only

For research use only

Target Details

Target

NFKB1

Official Name

NFKB1

Full Name

nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Alternative Names

NF-kB1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkappaB; p105; EBP-1; NF-kappa-B; NF-kappaB; NFKB-p50; KBF1; DNA-binding factor KBF1; p50; NFKB-p105