Human Phospho-NF-kappaB p65 (S468 / S536) / Total In-Cell ELISA Kit (Chemiluminescent) [Luminescent]
[CAT#: NPP2011ZP471]
- Applications:
- ELISA; In-Cell ELISA
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Product Overview
Description
The phosphp-nf-kappab p65 (S468 + S536) + Total In-Cell ELISA kit (Chemiluminescent) provides a simple, efficient, cell-based method for detecting phosphorylated activated protein. This kit is specifically designed to quantify activated (phosphorylated) NF-kappaB p65 and/or total NF-kappaB p65. Cells are cultured in 96-well plates and stimulated to induce pathways of interest. After stimulation, cells are quickly fixed to preserve activation-specific protein modifications. Each well is then incubated with a primary antibody that can recognize phosphorylated NF-kappaB p65 or total NF-kappaB p65. Subsequent incubation with hrp-labeled secondary antibody and developing solution provides a chemiluminescence readout that is easy to quantify. Then use the provided crystal violet solution to determine the relative number of cells per well. The 96-well plate format is suitable for high-throughput screening applications.
Species Reactivity
Human
Applications
ELISA; In-Cell ELISA
Research Areas
Neurodegenerative Diseases
Pathways
Alzheimer's Disease Signaling
Marker
Tangles & Tau
Properites
Platform
Microplate
Detection Method
Luminescent
Sample Type
Adherent cells, Suspension cells
Assay Type
Cell-based (quantitative)
Assay Duration
Multiple steps standard assay
Preparation and Storage
Storage
Store at +4°C. Please refer to protocols.
Research Use Only
For research use only
Target Details
Target
NFKB1
Official Name
NFKB1
Full Name
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Alternative Names
NF-kB1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkappaB; p105; EBP-1; NF-kappa-B; NF-kappaB; NFKB-p50; KBF1; DNA-binding factor KBF1; p50; NFKB-p105
Publications
Publications (0)
Neural Antibodies
Kits
Neural Genes
Case Studies
For Research Use Only. Not For Clinical Use.
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