Human S100 calcium binding protein A9/calgranulin B, S100A9 ELISA Kit

[CAT#: NRZP-0722-ZP234]

For the quantitative determination of human S100 calcium binding protein A9/calgranulin B (S100A9) concentrations in serum, plasma, tissue homogenates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human S100A9.

Species Reactivity

Human

Marker

S100 calcium binding protein A9

Properites

Sensitivity

In most cases, the sensitivity is below 1.17 ng/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, tissue homogenates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

4.69 ng/ml-300 ng/ml

Max Emission

450 nm

Target Details

Target

S100A9

Official Name

S100A9

Full Name

S100 calcium binding protein A9

Alternative Names

60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B), calgranulin B

Gene ID

6280(Human)

Uniprot ID

P06702(Human)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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