Mouse Protein S100-A9(S100A9) ELISA kit

[CAT#: NRZP-0722-ZP235]

For the quantitative determination of mouse protein S100-A9 (S100A9) concentrations in serum, plasma, tissue homogenates, cell culture supernates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of mouse S100A9.

Species Reactivity

Mouse

Marker

S100 calcium binding protein A9

Properites

Sensitivity

In most cases, the sensitivity is below 0.11 ng/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, cell culture supernates and tissue homogenates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

0.45 ng/ml-30 ng/ml

Max Emission

450 nm

Target Details

Target

S100A9

Official Name

S100A9

Full Name

S100 calcium binding protein A9

Alternative Names

60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B), calgranulin B

Gene ID

20202(Mouse)

Uniprot ID

P31725(Mouse)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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