Lenti of Rat adaptor-related protein complex 2, alpha 2 subunit (Ap2a2) (NM_031008) ORF clone, mGFP Tagged

Description

This is a rat AP2A2 mGFP tagged ORF clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

AP2A2

Species

Rat

Insert Size (bp)

2817 bp

Tag

mGFP

Type

Expression Vector/ Viral Particle

Vector

pLenti

E. coli Selection

Chloramphenicol (34 ug/mL)

Cell Selection

Puromycin

ORF Nucleotide Sequence

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGCCGGCCGTATCCAAGGGGGAGGGGATGCGAGGCCTAGCGGTCTTCATCTCCGATATCCGTAACTGTAAAAGTAAAGAAGCTGAAATAAAGAGGATAAATAAGGAGCTGGCAAATATTAGATCAAAATTTAAAGGTGACAAGGCTCTTGATGGCTACAGTAAGAAGAAGTATGTCTGCAAGTTACTCTTCATCTTTCTCCTTGGTCATGACATTGACTTTGGACACATGGAAGCTGTGAATCTTCTGAGCTCAAACAGATACACGGAAAAGCAGATCGGCTACCTTTTCATCTCTGTATTGGTGAATTCAAATAGTGAACTGATCCGGTTGATTAACAATGCCATCAAGAATGACCTGGCCAGCCGCAATCCTACATTTATGGGTCTGGCCCTGCACTGCATCGCCAATGTGGGTAGCCGTGAGATGGCAGAGGCCTTCGCTGGAGAGATCCCCAAGATCCTGGTGGCAGGAGATACCATGGACAGTGTGAAGCAGAGCGCAGCCCTGTGCTTGTTGCGCCTGTACAGGACGTCACCTGACCTAGTTCCTATGGGCGACTGGACATCCCGTGTGGTACACCTCCTCAATGACCAGCACTTGGGTGTAGTGACTGCCGCAACTAGCCTCATCACCACACTGGCCCAGAAGAATCCTGAGGAGTTCAAGACCTCTGTCTCTCTAGCTGTCTCTCGACTGAGCAGAATTGTGACGTCCGCGTCAACAGATCTTCAGGATTATACTTACTACTTTGTCCCGGCTCCTTGGCTGTCAGTCAAACTTCTGAGACTGCTGCAGTGCTACCCACCCCCAGAAGACCCTGCGGTGCGTGGCCGCCTGACTGAGTGCTTGGAGACCATCCTGAATAAGGCCCAGGAGCCCCCCAAGTCCAAGAAAGTCCAGCATTCAAATGCCAAGAACGCTGTGCTGTTTGAGGCCATCAGCCTCATCATCCACCATGACAGTGAGCCCAACCTCCTGGTCCGTGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCATCGGGAGACAAACCTGCGCTACCTGGCTCTGGAGAGCATGTGCACACTGGCCAGCTCTGAGTTCTCTCACGAGGCTGTCAAGACCCACATCGAGACTGTCATCAATGCCTTGAAGACGGAACGAGATGTGAGTGTGCGGCAGCGGGCAGTGGACCTGCTCTACGCCATGTGCGACCGAAGCAACGCCCAGCAGATTGTGGCTGAGATGCTGAGCTACCTAGAAACAGCTGACTACTCCATCCGGGAGGAAATTGTGCTGAAGGTGGCCATCCTTGCTGAGAAGTATGCAGTGGACTACACTTGGTATGTGGACACCATCCTCAACCTCATCCGCATTGCAGGCGACTATGTGAGCGAAGAGGTGTGGTACCGCGTCATCCAGATTGTCATCAATCGCGATGATGTGCAGGGTTATGCTGCCAAGACGGTGTTTGAGGCATTGCAGGCTCCAGCATGTCATGAGAACTTGGTCAAAGTGGGCGGCTACATCCTGGGCGAGTTTGGAAACTTGATAGCGGGAGACCCACGATCCAGCCCTCTGATCCAGTTCAACCTGCTCCACTCCAAGTTCCACTTGTGCAGCGTCCCCACTCGGGCACTCCTGCTGTCCACCTACATCAAGTTCGTGAACCTCTTTCCAGAGGTAAAGGCTACCATTCAGGACGTGCTGCGCAGTGACAGCCAACTGAAGAACGCAGATGTGGAGCTGCAGCAGCGGGCTGTGGAGTACCTGCGACTGAGTACTGTTGCCAGCACTGATATCCTGGCAACTGTCCTGGAAGAAATGCCACCCTTCCCTGAGCGTGAGTCCTCCATCTTGGCCAAGCTGAAGAAGAAGAAGGGCCCAAGCACAGTGACTGACCTGGAGGAGACCAAGCGTGAGCGAAGCATCGATGTGAATGGGGGCCCCGAACCCGTTCCAGCCAGCACCAGTGCTGCGTCCACACCTTCTCCATCAGCAGACCTGCTGGGTCTGGGGGCCGTTCCCCCTGCTCCCACAGGGCCCCCTCCCACCTCTGGCGGTGGGTTACTGGTGGACGTGTTCTCAGACTCAGCTTCTGCAGTTGCACCTCTTGCACCCGGCTCTGAAGACAACTTTGCCAGGTTTGTTTGTAAAAACAATGGTGTGTTGTTTGAAAACCAGCTGCTTCAAATTGGACTGAAGTCAGAATTTCGGCAGAATTTAGGTCGAATGTTCATATTTTATGGTAACAAGACCTCCACGCAGTTCCTAAACTTCACTCCAACACTAATCTGTGCTGACGACCTTCAGACTAATCTGAACCTGCAGACTAAGCCCGTGGACCCAACTGTGGATGGGGGCGCACAGGTGCAACAGGTGGTCAACATTGAGTGTATATCTGATTTCACAGAGGCACCTGTCCTCAACATCCAGTTCAGGTATGGTGGTACCTTCCAGAATGTGTCTGTTAAGCTTCCCATCACTCTCAACAAATTTTTCCAGCCCACAGAAATGGCTTCTCAGGATTTCTTTCAACGTTGGAAGCAGTTGAGCAATCCACAGCAAGAAGTGCAGAATATCTTCAAAGCAAAGCATCCGATGGACACAGAAATCACTAAAGCGAAGATTATTGGATTTGGTTCTGCGCTCCTGGAAGAAGTTGATCCAAACCCTGCAAATTTTGTGGGTGCTGGCATAATACATACCAAAACCACCCAGATCGGCTGCTTGTTGCGCCTGGAGCCAAACCTGCAAGCTCAGATGTACAGACTCACCTTGCGTACCAGCAAAGACACCGTCTCTCAGAGACTATGTGAATTGCTGTCGGAACAGTTCACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA

Protein Sequence

MPAVSKGEGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIANVGSREMAEAFAGEIPKILVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEETKRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPTSGGGLLVDVFSDSASAVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICADDLQTNLNLQTKPVDPTVDGGAQVQQVVNIECISDFTEAPVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKDTVSQRLCELLSEQFTRTRPLEQKLISEEDLAANDILDYKDDDDKV

Restriction Sites

SgfI-MluI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neural Signal Transduction

Relevant Diseases

Huntington's Disease

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at-20°C. The DNA is stable for at least one year from date of shipping when stored at-20°C

Research Use Only

For research use only

Target

AP2A2

Official Name

AP2A2

Full Name

AP-2 complex subunit alpha-2

Alternative Names

AP2A2, ADTAB, CLAPA2, HIP-9, HIP9, HYPJ, adaptor related protein complex 2 alpha 2 subunit, adaptor related protein complex 2 subunit alpha 2

Gene ID

161(Human); 11771(Mouse); 308578(Rat)

Uniprot ID

O94973(Human); P17426(Mouse); P18484(Rat)