Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric)

Description

Monoamine oxidase (MAO, EC 1.4.3.4) is a class of enzymes that can oxidize a variety of endogenous primary amines. Based on their substrate, inhibitor specificity and tissue localization, two isoforms have been identified, MAO-A and MAO-B. MAO-A can oxidize primary amines such as serotonin and norepinephrine. MAO-A is a mitochondrial binding enzyme, which is commonly expressed throughout the brain and other tissues. It is related to panic, anxiety and depression. Several MAO-A-specific inhibitors, such as periodine, flubutamine, toloxatone, tetrindole, etc., have been used as antidepressants, but their use is restricted due to side effects. Our MAO-A inhibitor screening kit provides a fast, simple, sensitive and reliable test method, suitable for high-throughput screening of MAO-A inhibitors. The determination is based on the fluorescence detection of H2O2, which is one of the by-products produced during the oxidative deamination of MAO substrates.
Screening/studying/characterizing MAO-A inhibitors

Relevant Diseases

Neurological Disorders

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Components

• MAO-A Assay Buffer
• OxiRed™ Probe (in DMSO)
• MAO-A Enzyme (Lyophilized)
• MAO-A Substrate (Lyophilized)
• Developer (Lyophilized)
• Inhibitor Control (Clorgyline) (Lyophilized)

Shipping

Gel Pack

Storage

-20°C

Research Use Only

For research use only

Target

Monoamine Oxidase A

Official Name

MAOA

Full Name

Monoamine oxidase A

Alternative Names

MAOA; MAO-A; monoamine oxidase A; BRNRS

Gene ID

4128(Human); 17161(Mouse); 29253(Rat)

Uniprot ID

P21397(Human); Q64133(Mouse); P21396(Rat)