Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric)

Description

Monoamine oxidase (MAO, EC 1.4.3.4) is a class of enzymes that can oxidize a variety of endogenous primary amines. Based on their substrate, inhibitor specificity and tissue localization, two isoforms have been identified, MAO-A and MAO-B. MAO-B can oxidize primary amines, but compared with MAO-A, the list of specific substrates (ie benzylamine, phenethylamine) is limited. MAO-B is a mitochondrial binding enzyme, which is ubiquitously expressed throughout the brain and other tissues. Many studies have studied it, including Parkinson's disease, Alzheimer's disease and tobacco addiction. Certain MAO-B inhibitors (such as selegiline and rasagiline) have been used to treat patients with Parkinson's disease, but their benefits are considered to be quite limited. Our MAO-B inhibitor screening kit provides a fast, simple, sensitive and reliable test method, suitable for high-throughput screening of MAO-B inhibitors. The measurement is based on the fluorescence detection of H2O2, which is one of the by-products produced during the oxidative deamination of MAO substrates.
Screening/studying/characterizing MAO-B inhibitors

Relevant Diseases

Neurological Disorders

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Components

• MAO-B Assay Buffer
• OxiRed™ Probe (in DMSO)
• MAO-B Enzyme (Lyophilized)
• MAO-B Substrate (Lyophilized)
• Developer (Lyophilized)
• Inhibitor Control (Clorgyline) (Lyophilized)

Shipping

Gel Pack

Storage

-20°C

Research Use Only

For research use only

Target

Monoamine Oxidase B

Official Name

MAOB

Full Name

Monoamine oxidase B

Alternative Names

MAOB; Monoamine oxidase B

Gene ID

4129(Human)

Uniprot ID

P27338(Human)