Mouse cAMP response element binding protein(CREB)ELISA Kit

Name: Mouse cAMP response element binding protein(CREB)ELISA Kit
SKU: NRZP-0722-ZP262
Rating: 5 (1 reviews)

[CAT#: NRZP-0722-ZP262]

For the quantitative determination of mouse cAMP response element binding protein (CREB) concentrations in serum, plasma, cell culture supernates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of mouse CREB.

Species Reactivity

Mouse

Marker

cAMP response element binding protein(CREB)

Properites

Sensitivity

In most cases, the sensitivity is below 0.78 pg/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, cell culture supernates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

3.12 pg/ml-200 pg/ml

Max Emission

450 nm

Target Details

Target

CREB

Official Name

CREB1

Full Name

cAMP responsive element binding protein 1

Alternative Names

Cyclic AMP-responsive element-binding protein 1; CREB; CREB-1; cAMP-responsive element-binding protein 1

Gene ID

12912(Mouse)

Uniprot ID

Q01147(Mouse)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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