NeuroMab™ Anti-PRNP BBB Shuttle Antibody,Clone NR1395P
- Host Species:
- Mouse
- Species Reactivity:
- Human
- Applications:
- IP; WB; ELISA; Inhib; In Vitro
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Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.
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Figure 1. Western blot of normal bovine brain homogenate and recombinant bovine PrP. mAb 6H4 and 34C9 recognize bovine PrP and rbPrP, whereas may 15B3 recognizes only rbPrP Recombinant rbPrP runs lower and more acutely than PrP from brain homogenate because it is not glycosylated.
Figure 2 Epitope mapping of the bovine PrP peptide library.
mAB 34C9 recognizes peptides Nos. 59 to 63 of the peptide library comprising the epitopes of amino acids 149 to 153 (Leu Ile His Phe Gly) of bovine PrP.
Figure 2 Epitope mapping of the bovine PrP peptide library.
mAB 6H4 recognizes peptides Nos. 64 to 66 of the peptide library comprising the epitopes of amino acids 155 to 163.
Figure 3. Immunoprecipitation of bovine, mouse and human PrP using mAbs 15B3 and 6H4
Proteinase K digestion of immunoprecipitated PrP BSE using mA 15B3. Undigested and digested bovine brain homogenates were compared to immunoprecipitates digested with protein A-agarose only or proteinase K with 15B3.
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