NeuroMab™ Hamster Anti-DLL1 Monoclonal Antibody (CBP1583)

Description

The antibody recognizes delta-like protein 1 (DLL1), one of the five main ligands of the Notch signaling pathway, and is activated by the binding of specific ligands to Notch receptors Notch 1-4. Notch signaling pathway is an evolutionary conserved pathway in multicellular organisms. It is essential for cell-to-cell communication in basic developmental and physiological processes (including regulation of neurons, heart and endocrine development, stem cell hematopoiesis, thymic T cell development, and Tumor progression and suppression. The Notch receptor connects with its specific ligands Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal-receiving cells, through the ADAM family of metalloproteinases and gamma- The protein induces proteolysis of the receptor. The secretin complex in the transmembrane domain releases the Notch intracellular domain (NICD), which translocates to the nucleus. Subsequent signal transduction occurs through the complex cascade of CSL-NICD-Mastermind (canonical pathway) or the complex signal cascade of NF-kappaB-NICD and CSL-NICD-Deltex (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, while the non-canonical pathway promotes differentiation.

Immunogen

DLL1-expressing CHO cells.

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Hamster

Isotype

IgG

Clone Number

CBP1583

Applications

FC; IHC-Fr

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1371

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Concentration

1 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein G.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

DLL1

Official Name

DLL1

Full Name

Delta Like Canonical Notch Ligand 1

Alternative Names

Delta Like Canonical Notch Ligand 1; Drosophila Delta Homolog 1; H-Delta-1; Delta1; Delta (Drosophila)-Like 1; Delta-Like 1 (Drosophila); Delta-Like Protein 1; Delta; DL1;

Gene ID

28514(Human); 13388(Mouse); 84010(Rat)

Uniprot ID

O00548(Human); Q61483(Mouse); P97677(Rat)

1. Moriyama, Y. et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. 2. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands. 3. Sekine, C. et al. (2012) Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes. 4. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex. 5. Iso, T. et al. (2003) Notch signaling in vascular development. 6. Hu, X. et al. (2008) Integrated regulation of Toll-like receptor responses by Notch and interferon-gamma pathways. 7. Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity.