Hamster Anti-Jag1 Monoclonal Antibody (CBP1584)

Description

The antibody specifically recognizes CD339 (also known as Jagged1), one of the five main ligands of the Notch signaling pathway, which is activated by the binding of specific ligands to Notch receptors Notch 1-4. Notch signaling pathway is an evolutionary conserved pathway in multicellular organisms. It is essential for cell-to-cell communication in basic developmental and physiological processes (including regulation of neurons, heart and endocrine development, stem cell hematopoiesis, thymic T cell development, and Tumor progression and suppression. Notch receptors are connected by their specific ligands Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, and are physically adjacent to signal-receiving cells through ADAM family metalloproteinases and γ-receptors Induces proteolysis of the receptor. The secretin complex in the transmembrane domain releases the Notch intracellular domain (NICD), which translocates to the nucleus. Then, subsequent signal transduction occurs through the CSL-NICD-Mastermind complex cascade (standard pathway) or the NF-kappaB-NICD and CSL-NICD-Deltex complex signal cascade (non-classical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, while the non-canonical pathway promotes differentiation.

Immunogen

Jagged1-expressing CHO cells.

Species Reactivity

Mouse; Rat

Clonality

Monoclonal

Host Species

Hamster

Isotype

IgG

Clone Number

CBP1584

Applications

FC; IHC-Fr

Research Areas

Growth and Development

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Concentration

1 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein G.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

CD339

Official Name

Jag1

Full Name

Jagged1

Alternative Names

JAG1; Jag1; ABE2; Gsfabe2; Htu; Ozz; Ser-1; AGS; AHD; AWS; CD339; HJ1; JAGL1; jagged 1; AGS1; DCHE; jagged canonical Notch ligand 1

Gene ID

182(Human); 16449(Mouse); 29146(Rat)

Uniprot ID

P78504(Human); Q9QXX0(Mouse); Q63722(Rat)

1. Moriyama Y et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. 2. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands. 3. Sekine, C. et al. (2012) Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes. 4. Koyanagi, A. et al. (2012) Expression of Notch receptors and ligands on immature and mature T cells. 5. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex. 6. Iso, T. et al. (2003) Notch signaling in vascular development. 7. Hu, X. et al. (2008) Integrated regulation of Toll-like receptor responses by Notch and interferon-gamma pathways. 8. Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity.