NeuroMab™ Mouse Anti-Huntingtin Monoclonal Antibody (CBP1945)

Product Overview

Description

The mouse anti-Huntingtin antibody clone HDB4E10 reacts with an epitope corresponding to the HDB region (amino acids 1844-2131) of the Huntingtin protein.
The mouse anti-Huntingtin antibody, clone HDB4E10, can detect a band of about 350 kDa on Western blot, but it can also detect smaller degradation products of huntingtin. The combined use of mouse anti-Huntingtin antibody, clone HDB4E10 (MCA2050) and HDC8A4 (MCA2051) proved that huntingtin is enriched in neuronal cells of the brain (Jones 1999).

Immunogen

Recombinant protein, corresponding to amino acids 1842-4131 of Huntingtin protein.

Species Reactivity

Human; Rat; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1945

Applications

IHC-Fr; WB; IP

Relevant Diseases

Huntington's Disease

Research Areas

Neuroregeneration

Product Properties

Form

Liquid

Formulation

PBS; 0.09% Sodium Azide

Preservatives

Yes

Concentration

1.0 mg/ml

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Huntingtin

Official Name

HTT

Full Name

huntingtin gene

Alternative Names

HTT; HD; IT15; huntingtin; LOMARS

Gene ID

3064(Human); 15194(Mouse); 29424(Rat)

Uniprot ID

P42858(Human); P42859(Mouse); P51111(Rat)

References

1. Shirasaki, D.I. et al. (2012) Network organization of the huntingtin proteomic interactome in mammalian brain.
2. Jones, A.L. (1999) The localization and interactions of huntingtin.
3. Tao, T. & Tartakoff, A.M. (2001) Nuclear relocation of normal huntingtin.
4. Massai, L. et al. (2013) Development of an ELISA assay for the quantification of soluble huntingtin in human blood cells.

Publications