Product
Mouse Anti-CTLA-4 Monoclonal Antibody (CBP2152)
[CAT#: NAB-2011-ZP139]
- Host Species:
- Mouse
- Species Reactivity:
- Human
- Applications:
- FC; ELISA
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryProduct Overview
Description
CTLA-4 (cytotoxic T-lymphocyte-4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. CTLA-4 is recruited from intracellular vesicles to the immunological synapse beginning 1-2 days after T cell activation. It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending T cell activation.
Immunogen
Recombinant human CTLA4-Fc (Z03373)
Species Reactivity
Human
Clonality
Monoclonal
Host Species
Mouse
Isotype
Mouse
Clone Number
CBP2152
Applications
FC; ELISA
Application Notes
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
ELISA blocking: 10-50 µg/ml
Flow cytometry: 2-10 µg/ml
Other Applications: user-optimized
The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
ELISA blocking: 10-50 µg/ml
Flow cytometry: 2-10 µg/ml
Other Applications: user-optimized
Conjugation
Unconjugated
Product Properties
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (This can be customized for bulk orders.)
Purification
Protein A affinity column
Shipping
The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Storage
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Handling Advice
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Target
CTLA-4
Official Name
CTLA4
Full Name
cytotoxic T-lymphocyte-associated protein 4
Alternative Names
GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12
Data
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
