Mouse Anti-CTLA-4 Monoclonal Antibody (CBP2154)
[CAT#: NAB-2011-ZP141]
- Host Species:
- Mouse
- Species Reactivity:
- Mouse
- Applications:
- ELISA
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SPECIFIC INQUIRY
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Description
Cytotoxic T-lymphocyte-associated protein 4 (CTLA4), also known as CD152, is one of the first well studied immune checkpoint molecules. CTLA4 is expressed on T cells and its expression level can be up-regulated after T cell activation. CTLA4 has two ligands CD80 (B7-1) and CD86 (B7-2). CTLA4 can compete with CD28 binding with these two ligands and transmits an inhibitory signal to T cells. Therapeutic antibodies against CTLA4 can help to stimulate T cells which can then target many cancer cells. Mouse CTLA-4 antibodies with has blocking effect against CD80/CD86 binding with CTLA4 can serve as a great surrogate antibody in mouse model.
Mouse CTLA-4 Antibody (MCT.F6), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from mouse immunized with recombinant mouse CTLA4-Fc
Mouse CTLA-4 Antibody (MCT.F6), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from mouse immunized with recombinant mouse CTLA4-Fc
Immunogen
Recombinant mouse CTLA-4-Fc
Species Reactivity
Mouse
Clonality
Monoclonal
Host Species
Mouse
Isotype
Mouse
Clone Number
CBP2154
Applications
ELISA
Application Notes
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
ELISA blocking: 10-50 µg/ml
Other Applications: user-optimized
The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
ELISA blocking: 10-50 µg/ml
Other Applications: user-optimized
Conjugation
Unconjugated
Product Properties
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (This can be customized for bulk orders.)
Purification
Protein A affinity column
Shipping
The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Storage
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Handling Advice
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Target
CTLA-4
Official Name
CTLA4
Full Name
cytotoxic T-lymphocyte-associated protein 4
Alternative Names
GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12
Data
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
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