Mouse Anti-LAG3 Monoclonal Antibody (CBP2157)

Description

LAG-3 (Lymphocyte activation gene-3), designated CD223, is a 70 kDa type I transmembrane protein that is a member of the immunoglobulin superfamily (IgSF). LAG-3 is expressed on activated CD4+ and CD8+ T cells, NK cells, and plasmacytoid dendritic cells (pDC), but not on resting T cells. LAG-3 on activated CD4+CD25+ Treg cells plays a role in their suppressive activity. LAG-3 limits the expansion of activated T cells and pDC in response to selected stimuli. In mice, deletion of LAG-3 and another negative regulator, PD-1, facilitates anti-cancer response but also blocks self-tolerance and increases susceptibility to autoimmune diseases. In humans, antibody-mediated down‑regulation of LAG-3 and PD-1 allows more effective control of chronic malaria, while in NOD (non‑obese diabetic) mice, deletion of LAG-3 alone accelerates diabetes.

Human LAG3 Antibody (LG.B5), mAb, Mouse is produced from a hybridoma resulting from the fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Recombinant human LAG3-Fc (R&D, 2319-L3).

Immunogen

Recombinant human LAG3-Fc (R&D, 2319-L3)

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

Mouse

Clone Number

CBP2157

Applications

FC; ELISA

Application Notes

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.



ELISA detection: 0.01-0.1 µg/ml

Flow cytometry: 2-10 µg/ml

Other Applications: user-optimized

Conjugation

Unconjugated

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (It could be customized for bulk order.)

Purification

Protein A affinity column

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.

Handling Advice

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

LAG3

Official Name

LAG3

Full Name

lymphocyte-activation gene 3

Alternative Names

CD antigen CD223; CD223; Protein FDC; Lymphocyte activation gene 3 protein; LAG-3

Gene ID

3902(Human);16768(Mouse)

Uniprot ID

P18627(Human);Q61790(Mouse)