Mouse Anti-Human SIRP Alpha Monoclonal Antibody (CBP2181)

Product Overview

Description

CD172a, also known as signal-regulatory protein α (SIRPα), is a 85-90 kD, type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. SIRPα is involved in the negative regulation of receptor tyrosine kinase-coupled signaling; the phospho-tyrosine residues of this protein recruit SH2 domain-containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. CD172a is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, neurons and by cardiomyocytes derived from human pluripotent stem cells. The ligands of CD172a are CD47, SP-A, and SP-D.

Immunogen

KG1a cells.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a, κ

Clone Number

CBP2181

Applications

Application Notes

FCM (5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

APC

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA.

Concentration

Batch Dependent

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Handling Advice

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

SIRP Alpha

Official Name

SIRPA

Full Name

Signal regulatory protein α

Alternative Names

BIT; MFR; P84; MYD-1; SHPS1; PTPNS1; CD172 antigen-like family member A

Gene ID

140885(Human); 19261(Mouse); 25528(Rat)

Uniprot ID

P78324(Human); P97797(Mouse); P97710(Rat)

References

1. Lv Z, et al. 2015. J. Immunol. 195:661.
2. Hatherley D, et al. 2014. J. Biol. Chem. 289:10024.
3. Zen K, et al. 2013. Nat. Commun. 4:2436.
4. Baba N, et al. 2013. J. Exp. Med. 210:1251.
5. Barclay AN, Brown MH. 2006. Nat. Rev. Immunol. 6:457.

Publications