Mouse Anti-SNAP25 Monoclonal Antibody (CBP2624)

Description

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.

Expression Host

Mammalian cells

Immunogen

SNAP25 fusion protein Ag6695

Species Reactivity

Human; Mouse; Rat; Porcine

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b

Clone Number

CBP2624

Applications

IF; IP; WB; ELISA

Application Notes

IF : 1:20-1:200; IP : 0.5-4.0 ug; WB : 1:500-1:1000

Research Areas

GPCR

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1613 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

SNAP-25

Official Name

SNAP25

Full Name

Synaptosomal-associated protein, 25kDa

Alternative Names

RIC 4; RIC4; SEC9; SNAP; SNAP 25; SNAP25; SUP; Super protein

Gene ID

6616(Human); 20614(Mouse); 25012(Rat)

Uniprot ID

P60880(Human); P60879(Mouse); P60881(Rat)

1. Duan, X. L., Guo, Z., He, Y. T., Li, Y. X., Liu, Y. N., Bai, H. H.,... & Suo, Z. W. (2020). SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain. Neuroscience, 429, 203-212. 2. Wang, Y. X., Li, Y. Z., Zhu, H. F., Zhang, Z. Y., Qian, X. L., & He, G. Y. (2020). STX2 drives colorectal cancer proliferation via upregulation of EXOSC4. Life Sciences, 118597. 3. Wang, Y., Xu, H., Jiao, H., Wang, S., Xiao, Z., Zhao, Y.,... & Li, T. (2018). STX2 promotes colorectal cancer metastasis through a positive feedback loop that activates the NF-κB pathway. Cell death & disease, 9(6), 1-14.