Mouse Anti-NANOG Monoclonal Antibody (CBP2934)

Product Overview

Description

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells.

Expression Host

Mammalian cells

Immunogen

NANOG fusion protein Ag21364

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2934

Applications

WB; ELISA

Application Notes

WB : 1:500-1:1000

Research Areas

Neuroendocrinology

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1000 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

NANOG

Official Name

NANOG

Full Name

Nanog homeobox

Alternative Names

hNanog; Homeobox protein NANOG; NANOG; Nanog homeobox

Gene ID

79923(Human)

Uniprot ID

Q9H9S0(Human)

References

1. Gao, L., Sang, J. Z., & Cao, H. (2019). Limonin enhances the radiosensitivity of nasopharyngeal carcinoma cells via attenuating Stat3-induced cell stemness. Biomedicine & Pharmacotherapy, 118, 109366.

Publications